REACTIVE OXYGEN USE IN BIOFILM CONTROL

描述（改编自申请人的摘要）：该项目涉及 牙科需要补救水污染问题，即与 牙科综合治疗台水管线内的生物膜形成，并达到低水平 在非手术用水点使用时会变脏。拟议的研究将 开发两种创新的经济方法， 化学反应性的有效杀生物剂，其可用于处理 (1)供水中自由流动的微生物生物负荷;以及（2）所附的 存在于水管中的生物膜。前者使用一种新型的生物杀灭剂进行攻击， 水处理“过滤器”杀死有机体。后者通过小说受到攻击 使用两种通用消毒剂， 杀生物剂这些技术的主要特点是易于实现， 目前推荐的牙科实践包括改装，方便使用， 连续过滤，过滤器上没有生物膜积聚， 对残留物或物质的潜在危害或有害的担忧 患者、工人、牙科材料或器械。第一阶段的目标 研究是为了证明实施的可行性，以减少牙科 单位水比ADA建议的水平低。研究计划涉及 开发和表征生产原型测试的技术 模型这些原型将用于评估微生物杀灭 在不同的条件下。 拟定商业应用： 这项技术的市场潜力是巨大的。 有超过150，000名牙医 仅在美国，ADA建议水污染 这个在牙科诊所普遍存在的问题，在2000年之前得到纠正。 市场 外部牙科甚至更大，包括其他医疗实践，制药和 食品工业和各种其他行业（生物污染相关成本> 10亿美元/年） 年）。

DESCRIPTION (Adapted from Applicant's Abstract): This project addresses dentistry's needs to remedy water contamination problems, namely related to biofilm formation within dental unit waterlines, and achieve low level bioburdens in point of use nonsurgical water. The proposed research will develop two innovative economical approaches of generating short-lived chemically reactive potent biocides useful for treating both the problems of (1) free flowing microbial bio-burden in water supplies; and (2) the attached biofilm resident in waterlines. The former is attacked using a novel biocidal water treatment "filter" to kill organisms. The latter is attacked via novel deployment of two common generic disinfectants to engender a third potent biocide. Key features of the techniques are their ease of implementation to current recommended dental practices including retrofitting, convenient use, continuous filtration with no bio-film buildup on the filter, and minimal concerns of residuals or substances potentially hazardous or harmful to patients, workers, dental materials or instruments. The objective of Phase I research is to demonstrate the feasibility of implementation to reduce dental unit water bioburdens below ADA recommended levels. The research plan addresses development and characterization of the technologies to produce prototypic test models. These prototypes will then be used to evaluate microorganism killing under various conditions. PROPOSED COMMERCIAL APPLICATION: The maket potential for the technology is enormous. there are over 150, 000 dental practices in the United States alone and the ADA recommends that the water contamination problem, essentially ubiquitous in dental clinics, be corrected by the year 2000. The market outside dentistry is even larger, encompassing other medical practices, pharmaceutical and food industries and a wide range of other commerces (biofouling related costs > $1 billion/ year).