ENZYMOLOGY OF EGGSHELL FORMATION IN SCHISTOSOMA MANSONI

曼氏血吸虫蛋壳形成的酶学研究

• 批准号: 2614898
• 负责人: DAVID LEE WILLIAMS
• 金额: $ 9.76万
• 依托单位: ILLINOIS STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-03-01 至 2002-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2614898
• 关键词: NAD(H) phosphate; Schistosoma mansoni; colorimetry; crosslink; egg shell; enzyme activity; immunocytochemistry; molecular cloning; monophenol monooxygenase; oogenesis; peroxidases; polymerase chain reaction; protein biosynthesis; protein tyrosine kinase; tyrosine 3 monooxygenase

The long term objective of this lab is to study development in Schistosoma mansoni, especially factors affecting the development of female worms. A central need of these studies is a marker to assess the developmental status of female worms. The enzymes involved in eggshell protein activation and cross linking are only expressed in adult, sexually active female worms. Enzymatic assays and molecular probes will be developed to monitor the expression of these enzymes. These enzymes will therefore serve as markers for female worm development. A parallel objective of this lab is to apply the knowledge gained in fundamental investigations of worm biochemistry and molecular biology to the generation of novel methods of schistosomiasis control. Schistosome eggs are of central importance in the pathology and transmission of schistosomiasis. A better understanding of the biochemical and molecular events involved in the production of eggs in the female schistosome may also lead to the development of novel chemotherapies or a schistosome vaccine. The specific research proposed in this application is to identify the enzymes involved in eggshell formation in female S. mansoni. Our working hypothesis is that eggshell formation is schistosomes uses enzymatic pathways similar to those used for eggshell formation and cuticle hardening by arthropods and mollusks; the enzymes of quinone tanning and Beta-sclerotization. The role that tyrosine hydrolase, phenol oxidase, and peroxidase play in schistosome eggshell production will be investigated. The goals of this project are 1) to determine role of peroxidase in eggshell formation; 2) to determine the role of tyrosine hydroxylase in the hydroxylation of schistosome eggshell proteins; 3) to determine the relationship of schistosome phenol oxidase to the phenol oxidases of other organisms by cloning its gene; 4) to understand the role of phenol oxidase in the hydroxylation and oxidation of schistosome eggshell proteins; and 5) to determine the stage and tissue specific expression phenol oxidase in schistosomes.

该实验室的长期目标是研究发展 曼氏血吸虫，特别是影响其发展的因素 雌虫。 这些研究的一个核心需求是评估一个标记 雌性蠕虫的发育状况。 蛋壳中涉及的酶 蛋白质激活和交联仅在成人中表达， 性活跃的雌性蠕虫。 酶测定和分子探针 将开发用于监测这些酶的表达。 这些 因此，酶将作为雌性蠕虫发育的标记。 该实验室的一个并行目标是应用在 蠕虫生物化学和分子生物学的基础研究 促进血吸虫病控制新方法的产生。 血吸虫卵在病理学中具有核心重要性， 血吸虫病的传播。 更好地理解 与鸡蛋生产有关的生化和分子事件 女性血吸虫也可能导致小说的发展 化疗或血吸虫疫苗。 提出的具体研究 该应用的目的是鉴定蛋壳中涉及的酶 雌性曼氏沙门氏菌的形成。 我们的工作假设是蛋壳 血吸虫的形成使用与所用类似的酶促途径 用于节肢动物和软体动物的蛋壳形成和角质层硬化； 醌鞣制和β-硬化酶。 所扮演的角色 酪氨酸水解酶、酚氧化酶和过氧化物酶在血吸虫中发挥作用 将调查蛋壳生产情况。 该项目的目标是 1) 确定过氧化物酶在蛋壳形成中的作用； 2）到 确定酪氨酸羟化酶在羟基化中的作用 血吸虫蛋壳蛋白； 3）确定关系 血吸虫酚氧化酶转化为其他生物体的酚氧化酶 克隆其基因； 4）了解酚氧化酶的作用 血吸虫蛋壳蛋白的羟基化和氧化； 5) 到 确定酚氧化酶的阶段和组织特异性表达 血吸虫。