EUKARYOTIC BIOLUMINESCENT INTEGRATED CIRCUIT SENSORS

真核生物发光集成电路传感器

• 批准号: 2870408
• 负责人: GARY S SAYLER
• 金额: $ 10.66万
• 依托单位: UNIVERSITY OF TENNESSEE KNOXVILLE
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-09-30 至 2000-09-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2870408
• 关键词: artificial endocrine pancreas; bioluminescence; biosensor device; biotechnology; cell line; cytodiagnosis; eukaryote; gene expression; glucose; patient monitoring device; plasmids; transfection

DESCRIPTION (Adapted from applicant's abstract): Current glucose biosensor technology relies on immobilized enzyme-based glucose oxidase bioreactors which have proven unreliable due to the presence of a glucose oxidase inhibitor in the human bloodstream. A biosensor utilizing who cells rather than just cellular constituents may prove more reliable for glucose monitoring. Additionally, white-light detection in response to glucose concentrations. Eukaryotic bioluminescent cell line containing the luxAB gene from Xenorhabadus luminescens instead of the convention luc genetic systems found in all other bioluminescent eukaryotes. In so doing, we will produce a eukaryotic cell line capable of monitoring glucose continuously, on-line and in real-time. The luxAB gene will be placed in pLPK.LucFF, a plasmid-based luc genetic system capable of sensing glucose. Once successfully constructed and tested for glucose sensitivity, the cells will be encapsulated on an integrated circuit containing a photodetector that will monitor the luminescence activity. The ultimate goal is to produce a reliable glucose sensor small enough to be used for routine implantation in diabetic patients.

描述（改编自申请人摘要）：当前葡萄糖生物传感器 该技术依赖于基于固定化酶的葡萄糖氧化酶生物反应器 由于存在葡萄糖氧化酶 人体血液中的抑制剂利用WHO细胞的生物传感器， 而不仅仅是细胞成分可能证明对葡萄糖更可靠 监测.此外，响应于葡萄糖的白光检测 浓度的含有luxAB的真核生物发光细胞系 该基因来源于发光小斑蝶，而不是传统的luc基因 在所有其他生物发光真核生物中发现的系统。在这样做时，我们将 产生能够连续监测葡萄糖的真核细胞系， 在线和实时。将luxAB基因置于pLPK中。 能够感测葡萄糖的基于质粒的luc遗传系统。一旦 成功构建并测试了葡萄糖敏感性， 将被封装在包含光电探测器的集成电路上 它将监测发光活动。最终目标是 生产一种可靠的葡萄糖传感器， 植入糖尿病患者体内。