HOMOGENEOUS GLYCOPROTEINS FOR STRUCTURAL BIOLOGY

用于结构生物学的均质糖蛋白

• 批准号: 2685162
• 负责人: TIMOTHY M LOGAN
• 金额: $ 10.12万
• 依托单位: FLORIDA STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-08-01 至 2000-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2685162
• 关键词: cell line; gene expression; glycoprotein structure; glycoproteins; glycosylation; method development; recombinant proteins; tissue /cell culture

DESCRIPTION: This high risk/high impact proposal seeks to demonstrate a method of producing recombinant glycoproteins with defined carbohydrate patterns and high homogeneity by expression in Lec1 CHO cells. These cells lack a key enzyme, and they produce proteins homogeneously glycosylated with N-acetylglucosamine2-mannose5. The approach is to be demonstrated with Thy-1 a 111 amino acid, 3 N-linked glycosylated surface glycoprotein that inhibits the growth of CNS neurons. The pEE14 glutamine synthetase based vectors would be used to provide enough Thy-1 for structure determination. The long-term gaol is to determine the structure of Thy-1 to gain insights into cell growth regulation in the CNS by this family of inhibitory surface glycoproteins. The hope is to facilitate the development of agents to promote CNS regeneration.

描述：这个高风险/高影响的提案试图证明一个