HOMOGENEOUS GLYCOPROTEINS FOR STRUCTURAL BIOLOGY

描述：这份高风险/高影响的提案旨在展示 一种生产含有特定碳水化合物的重组糖蛋白的方法 在Lec1 CHO细胞中表达的模式和高度的同源性。这些细胞 缺乏一种关键酶，它们会产生均一种糖基化的蛋白质 含N-乙酰氨基葡萄糖2-甘露糖5。这种方法有待演示。 用THY-1a 111个氨基酸，3个N-连接的糖基化表面糖蛋白 这会抑制中枢神经系统神经元的生长。PEE14谷氨酰胺合成酶 将使用基于载体的方法来为结构提供足够的Thy-1 决心。长期的目标是确定Thy-1的结构 通过这一家族对中枢神经系统中细胞生长调控的洞察 抑制性表面糖蛋白。希望是为了促进 开发促进中枢神经系统再生的药物。

DESCRIPTION: This high risk/high impact proposal seeks to demonstrate a method of producing recombinant glycoproteins with defined carbohydrate patterns and high homogeneity by expression in Lec1 CHO cells. These cells lack a key enzyme, and they produce proteins homogeneously glycosylated with N-acetylglucosamine2-mannose5. The approach is to be demonstrated with Thy-1 a 111 amino acid, 3 N-linked glycosylated surface glycoprotein that inhibits the growth of CNS neurons. The pEE14 glutamine synthetase based vectors would be used to provide enough Thy-1 for structure determination. The long-term gaol is to determine the structure of Thy-1 to gain insights into cell growth regulation in the CNS by this family of inhibitory surface glycoproteins. The hope is to facilitate the development of agents to promote CNS regeneration.