NUCLEAR PORE COMPLEX STRUCTURE/FUNCTION

这笔赠款的重点是确定两个已识别基因的特征； 前面描述的分化肌动蛋白、ACT2和小说 必需基因，NNF1。有证据表明，不同的行动， Act2p是合适的核孔复合体结构所必需的，并且 NNF1p在核膜形态上有缺陷，纺锤体 定向和信使核糖核酸输出。这些基因将被用来 了解更多关于核孔复合体的组织 以及鼻咽癌内特定蛋白质在 核质运输方面，应注意以下问题 地址：1)Act2p在哪里？2)NPC机构是否变更 在ACT2-1突变体中？3)Act2p、Nuplp和 Srplp直接？4)还有哪些蛋白质与Act2p相互作用？)是不是ATP 水解对Act2p功能重要吗？6)NIP位于哪里？ 7)nnf1t5等位基因分析。8)Nnflp是否会影响 核质GTP酶周期？基因上有哪些蛋白质 与Nnflp互动？

This grant focuses on the characterization of two identified genes; the previously described divergent actin, ACT2 and the novel essential gene, NNF1. There is evidence that the divergent action, Act2p, is required for proper nuclear pore complex structure and that Nnf1p has a defect in nuclear envelope morphology, spindle orientation, and mRNA export. These genes will be used to understand more about the organization of the nuclear pore complex and the role of specific proteins within the NPC in nucleocytoplasmic transport, The following questions are to be addressed: 1)Where is Act2p Located? 2) Is NPC organization altered in act2-1 mutants? 3) Is the interaction between Act2p, Nuplp and Srplp direct? 4)What other proteins interact with Act2p?) Is ATP hydrolysis important for Act2p function? 6) Where is Nip Located? 7)Analysis of nnf1t5 alleles. 8) Does Nnflp affect the nucleocytoplasmic GTPase cycle? 9) What proteins genetically interact with Nnflp?