NUCLEAR PORE COMPLEX STRUCTURE/FUNCTION

这项资助的重点是两个已确定的基因的特征; 先前描述的趋异肌动蛋白，ACT 2和新的 必需基因NNF 1。有证据表明，分歧行动， Act 2 p是适当的核孔复合体结构所必需的， Nnf 1 p在核膜形态、纺锤体 定向和mRNA输出。这些基因将用于 了解更多关于核孔复合体的组织 以及NPC中特定蛋白质在 核质转运，以下问题将被 问题：1）Act 2 P在哪里？2)全国人大组织是否变更 在act 2 -1突变体中？3)Act 2 p、Nuplp和 Srplp直接？4)哪些蛋白质与Act 2 p相互作用？）是ATP 水解对Act 2 p功能重要吗？6)Nip在哪里？ 7)nnf 1 t5等位基因分析。8)Nnflp是否影响 核质GT3循环 9)哪些蛋白质基因 与Nnflp互动？

This grant focuses on the characterization of two identified genes; the previously described divergent actin, ACT2 and the novel essential gene, NNF1. There is evidence that the divergent action, Act2p, is required for proper nuclear pore complex structure and that Nnf1p has a defect in nuclear envelope morphology, spindle orientation, and mRNA export. These genes will be used to understand more about the organization of the nuclear pore complex and the role of specific proteins within the NPC in nucleocytoplasmic transport, The following questions are to be addressed: 1)Where is Act2p Located? 2) Is NPC organization altered in act2-1 mutants? 3) Is the interaction between Act2p, Nuplp and Srplp direct? 4)What other proteins interact with Act2p?) Is ATP hydrolysis important for Act2p function? 6) Where is Nip Located? 7)Analysis of nnf1t5 alleles. 8) Does Nnflp affect the nucleocytoplasmic GTPase cycle? 9) What proteins genetically interact with Nnflp?