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CORE--MORPHOLOGY AND TISSUE CULTURE

CORE--MORPHOLOGY AND TISSUE CULTURE
核心——形态学和组织培养
批准号：
6273755
负责人：
JOHN RICHARD SLADEK
金额：
$21.53万
依托单位：
YALE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
1998
资助国家：
美国
项目状态：
已结题
起止时间：
1998-05-01 至 1999-04-30
来源：
https://reporter.nih.gov/project-details/6273755
关键词：
Cercopithecidae Parkinson's disease biomedical facility brain computer simulation dopamine histology immunocytochemistry immunofluorescence technique methylphenyltetrahydropyridine nervous system transplantation tissue /cell culture

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The morphology and tissue culture core will provide histological and tissue culture support for all three projects. Immunohistochemistry for tyrosine hydroxylase will be used as a primary marker for dopaminergic neurons. Quantitation of dopamine neuron numbers in grafted tissue and in the substantia nigra of MPTP treated monkeys will be assessed for each relevant experiment. Computer assisted three-dimensional reconstructions will be made, particularly for attempts at circuit reconstruction using striatal- nigral co-grafts. Additional morphological methods and techniques provided by this core and its personnel are cryopreservation, fluorescence histochemistry, riboprobe in situ hybridization for tyrosine hydroxylase, immunochemistry for a number of other transmitters. Core personnel perform all fetal brain dissections and participate in adult brain dissections and micropunches that rely upon a combined histochemical/biochemical procedure. The tissue culture facility will carry out pharmacologic studies described in Project 1.
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Nigrostriatal Tract Reconstruction
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    $19.91万
  • 财政年份：
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  • 负责人：
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  • 依托单位：
    UNIVERSITY OF COLORADO DENVER
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  • 负责人：
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  • 依托单位：
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9th Internatl Meeting on Neural Transplantation & Repair
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    6944557
  • 项目类别：
  • 资助金额：
    $2.3万
  • 财政年份：
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  • 负责人：
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    UNIVERSITY OF COLORADO DENVER
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  • 项目类别：
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  • 财政年份：
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  • 负责人：
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  • 依托单位：
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