CORE--MORPHOLOGY AND TISSUE CULTURE

形态学和组织培养核心将提供组织学和组织 三是文化支持。 酪氨酸免疫组织化学 羟化酶将被用作多巴胺能神经元的主要标志物。 移植组织和移植组织中多巴胺神经元数量的定量 将评估MPTP处理猴的黑质中的每种相关 实验 计算机辅助三维重建将是 特别是在电路重建使用纹状体的尝试， 黑质共同移植物。 提供了其他形态学方法和技术 由这个核心和它的人员是冷冻保存，荧光 组织化学，酪氨酸羟化酶核糖探针原位杂交， 免疫化学检测其他一些递质。 核心人员执行 所有胎儿脑解剖和参与成人脑解剖， 微穿孔器依赖于组合的组织化学/生物化学程序。 组织培养设施将进行药理学研究， 在项目1中。

The morphology and tissue culture core will provide histological and tissue culture support for all three projects. Immunohistochemistry for tyrosine hydroxylase will be used as a primary marker for dopaminergic neurons. Quantitation of dopamine neuron numbers in grafted tissue and in the substantia nigra of MPTP treated monkeys will be assessed for each relevant experiment. Computer assisted three-dimensional reconstructions will be made, particularly for attempts at circuit reconstruction using striatal- nigral co-grafts. Additional morphological methods and techniques provided by this core and its personnel are cryopreservation, fluorescence histochemistry, riboprobe in situ hybridization for tyrosine hydroxylase, immunochemistry for a number of other transmitters. Core personnel perform all fetal brain dissections and participate in adult brain dissections and micropunches that rely upon a combined histochemical/biochemical procedure. The tissue culture facility will carry out pharmacologic studies described in Project 1.