CORE--MORPHOLOGY AND TISSUE CULTURE

形态和组织培养核心将提供组织学和组织学 为所有三个项目提供文化支持。酪氨酸的免疫组织化学研究 羟基酶将作为多巴胺能神经元的主要标志物。 移植组织中多巴胺神经元数量的定量测定 MPTP处理的猴子的黑质将被评估为每个相关的 做实验。计算机辅助的三维重建将是 尤其是对于尝试使用纹状体重建电路- 黑质联合移植。提供了其他形态方法和技术 由这个核心和它的人员是冷冻保存，荧光 组织化学、酪氨酸羟基酶的核探针原位杂交 其他一些传播者的免疫化学。核心员工表现 所有胎儿脑解剖和参与成人脑解剖和 依靠组织化学/生物化学相结合的程序的微型冲床。 组织培养设施将进行所述的药理学研究 在项目1中。

The morphology and tissue culture core will provide histological and tissue culture support for all three projects. Immunohistochemistry for tyrosine hydroxylase will be used as a primary marker for dopaminergic neurons. Quantitation of dopamine neuron numbers in grafted tissue and in the substantia nigra of MPTP treated monkeys will be assessed for each relevant experiment. Computer assisted three-dimensional reconstructions will be made, particularly for attempts at circuit reconstruction using striatal- nigral co-grafts. Additional morphological methods and techniques provided by this core and its personnel are cryopreservation, fluorescence histochemistry, riboprobe in situ hybridization for tyrosine hydroxylase, immunochemistry for a number of other transmitters. Core personnel perform all fetal brain dissections and participate in adult brain dissections and micropunches that rely upon a combined histochemical/biochemical procedure. The tissue culture facility will carry out pharmacologic studies described in Project 1.