DEVELOPMENT OF THE SENSE OF TASTE

味觉的发展

• 批准号: 2612817
• 负责人: CHARLOTTE M MISTRETTA
• 金额: $ 26.7万
• 依托单位: UNIVERSITY OF MICHIGAN AT ANN ARBOR
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-02-01 至 2003-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2612817
• 关键词: afferent nerve; cell type; developmental neurobiology; electrophysiology; embryo /fetus cell /tissue; ganglions; innervation; laboratory rat; mesenchyme; mixed tissue /cell culture; neurogenesis; neurotrophic factors; organ culture; taste buds; tongue; vertebrate embryology

DESCRIPTION: (Adapted From the Applicants Abstract): This proposal deals with the role of several neurotrophic factors in the fetal development of sensory neurons that innervate taste buds, and interactions between fetal cell types in the morphogenesis of mature taste organs. The P.I. will be able to use an organ culture system developed in her laboratory as an essential resource for all these goals. Firstly, the role of neurotrophic factors in supporting the development of the neurons the innervate taste buds have a different neurotrophic dependence than neurons from other sensory ganglia. Such dependence will be measured in terms of morphometric studies on neurite outgrowth and in terms of the development of critical electrophysiological properties in the sensory neurons. Secondly, the organ culture system will allow detailed analyses of any potential induction roles that embryonic tongue epithelium and mesenchyme may play in the formation of gustatory papillae. Finally, the interactions of the growing neurites of sensory neurons with developing taste buds will be examined. The later two aims will be approached by establishing mixed cultures of epithelium and mesenchyme or papillae and ganglia of different ages and morphogenetic potential. These data will form a significant body of knowledge about the development of the peripheral gustator system.

描述：（改编自申请人摘要）：本提案涉及 几种神经营养因子在胎儿发育中的作用 感觉神经元，支配味蕾，和胎儿之间的相互作用， 成熟味觉器官形态发生中的细胞类型。私家侦探将 能够使用她实验室开发的器官培养系统作为 这是实现所有这些目标的重要资源。第一，神经营养的作用 支持神经元发育的因素 芽有不同的神经营养依赖比神经元从其他 感觉神经节这种依赖性将在形态测量方面进行测量。 关于神经突生长的研究， 感觉神经元的电生理特性。二是 器官培养系统将允许详细分析任何潜在的 胚胎舌上皮细胞和间充质细胞诱导作用 味觉乳头的形成最后， 随着味蕾的发育， 考察后两个目标将通过建立混合 上皮和间充质或乳头和神经节的不同培养物 年龄和形态发生潜力。这些数据将构成一个重要的主体 周边味觉系统发展的知识。