DEVELOPMENT OF THE SENSE OF TASTE

味觉的发展

• 批准号: 2430084
• 负责人: CHARLOTTE M MISTRETTA
• 金额: $ 22.69万
• 依托单位: UNIVERSITY OF MICHIGAN AT ANN ARBOR
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-02-01 至 1998-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2430084
• 关键词: afferent nerve; developmental neurobiology; dietary sodium; embryo /fetus cell /tissue; ganglions; innervation; laboratory rat; mixed tissue /cell culture; neuroanatomy; neurogenesis; neurons; organ culture; solitary tract nucleus; taste; taste buds; tongue; trigeminal nerve; vertebrate embryology

DESCRIPTION (Adapted from the Investigator's Abstract): The proposal deals with very early development of peripheral and central taste systems in the rat. Three of the specific aims build on the principal investigator's demonstration that taste papillae will form in vitro from organ cultures of the embryonic rat tongue lacking normal sensory innervation. Specific Aim 1 will determine the potential for development in organ culture as a function of the age of the embryonic tissue. It will attempt to establish embryo age thresholds and cut- offs. Specific Aim 2 determines if in vivo molecular characteristics of tongue are maintained in organ culture. Aim 3 determines the effects of co-culturing the tongue with gustatory and nongustatory sensory ganglia on papilla and taste-bud formation in vitro. Specific aims 4 nd 5 deal with second order taste neuron development and utilize brainstem slices of the NST to explore basic changes in neuron circuitry and function. Specific Aim 4 tests the hypothesis that the adult level of sensitivity of NST neurons to excitatory and inhibitory neurotransmitters is acquired gradually following birth. Aim 5 tests the hypothesis that intrinsic neurotransmitter characteristics of rat NST cells are altered by dietary sodium deprivation. These in vitro systems have not previously been used to study development in taste, a sense that influences food selection and nutrition.

描述（改编自研究者摘要）：该提案