GENETIC ANALYSIS OF MESODERM INDUCTION IN MICE

小鼠中胚层诱导的遗传分析

基本信息

项目摘要

DESCRIPTION (adapted from investigator's abstract): This is a revised application to study the function of the msd locus in mouse development. The msd mutant phenotype consists of a failure to form mesoderm and the locus is defined by a series of overlapping deletions within the albino complex. The broad goal of the proposed research is to understand the genetic regulation of mesoderm differentiation. The specific aims of the proposal are to 1) determine if the msd mutation disrupts growth factor signaling. 2) To assess whether msd has other roles during development besides its involvement in mesoderm formation, and 3) to clone the msd gene. For specific aim 1, marked wild type ES cells will be injected into msd mutant blastocysts to determine whether msd endoderm is able to produce a mesoderm inducing signal(s). In addition, the expression patterns of Nodal, BMP-4 and Bmpr will be examined in mutant msd embryos to determine if msd functions by blocking production or reception of known mesoderm inducing signals. In a related series of experiments, regulated ectopic expression of known downstream components of mesoderm signaling pathways such as Mad1 , T and Map kinase will be examined for their ability to bypass the mesoderm block in msd mutants. In section 2 additional characterization of the msd phenotype will include experiments to address the cell-cycle length during early gastrulation, in situ hybridization studies to determine if early markers of anteroposterior axis formation are expressed in msd mutant embryos, production of chimeric embryos containing mutant msd cells in a wildtype background, and isolation of new ENU induced mutations in the msd gene. In the third section of the grant, approaches to clone the msd region are described. They include the isolation of a contig spanning the msd region and identification of transcription units within the contig by cDNA selection. The msd gene or genes will be identified by rescue of mutant embryonic stem cells after transfection with either individual cDNA or multiple cDNA clones.
描述(改编自研究者摘要):这是一篇经过修订的文章

项目成果

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BERNADETTE C HOLDENER其他文献

BERNADETTE C HOLDENER的其他文献

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{{ truncateString('BERNADETTE C HOLDENER', 18)}}的其他基金

Role of POGLUT2 and POGLUT3 in regulating microfibril structure and function
POGLUT2和POGLUT3在调节微纤维结构和功能中的作用
  • 批准号:
    10636927
  • 财政年份:
    2022
  • 资助金额:
    $ 24.21万
  • 项目类别:
Role of Beta3-Glucosyltransferase in a non-canonical quality control pathway
Beta3-葡萄糖基转移酶在非规范质量控制途径中的作用
  • 批准号:
    10427381
  • 财政年份:
    2018
  • 资助金额:
    $ 24.21万
  • 项目类别:
Role of Beta3-Glucosyltransferase in a non-canonical quality control pathway
Beta3-葡萄糖基转移酶在非规范质量控制途径中的作用
  • 批准号:
    10221012
  • 财政年份:
    2018
  • 资助金额:
    $ 24.21万
  • 项目类别:
Role of Beta3-Glucosyltransferase in a non-canonical quality control pathway
Beta3-葡萄糖基转移酶在非规范质量控制途径中的作用
  • 批准号:
    9579777
  • 财政年份:
    2018
  • 资助金额:
    $ 24.21万
  • 项目类别:
The biochemical mechanism of Wnt signaling
Wnt信号的生化机制
  • 批准号:
    7171930
  • 财政年份:
    2003
  • 资助金额:
    $ 24.21万
  • 项目类别:
Biochemical and genetic analysis of mesd function
med功能的生化和遗传分析
  • 批准号:
    6921930
  • 财政年份:
    1997
  • 资助金额:
    $ 24.21万
  • 项目类别:
Biochemical and genetic analysis of mesd function
med功能的生化和遗传分析
  • 批准号:
    7194947
  • 财政年份:
    1997
  • 资助金额:
    $ 24.21万
  • 项目类别:
GENETIC ANALYSIS OF MESODERM INDUCTION IN MICE
小鼠中胚层诱导的遗传分析
  • 批准号:
    6386306
  • 财政年份:
    1997
  • 资助金额:
    $ 24.21万
  • 项目类别:
GENETIC ANALYSIS OF MESODERM INDUCTION IN MICE
小鼠中胚层诱导的遗传分析
  • 批准号:
    2701719
  • 财政年份:
    1997
  • 资助金额:
    $ 24.21万
  • 项目类别:
GENETIC ANALYSIS OF MESODERM INDUCTION IN MICE
小鼠中胚层诱导的遗传分析
  • 批准号:
    6181299
  • 财政年份:
    1997
  • 资助金额:
    $ 24.21万
  • 项目类别:

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