REGULATION OF SELENOPROTEIN RNA METABOLISM

描述：拟议研究的具体目标是扩大 了解哺乳动物细胞中硒蛋白RNA的代谢。但 研究人员的发现可能适用于其他无意义的 转录本，并提供重要的洞察顺式作用序列， 反式作用因子促进或抑制无义介导的mRNA降解 （NMD）。具体目标是：1.深入了解 GPx 1 mRNA翻译在Sec 密码子介导细胞质mRNA衰变。2.确定提前终止的原因 在Sec密码子位置的PHGPx mRNA翻译不能介导 PHGPx mRNA丰度显著降低。

DESCRIPTION: The specific goal of the proposed research is to expand the understanding of selenoprotein RNA metabolism in mammalian cells. However, the investigator's findings will likely apply to other nonsense-containing transcripts and lend important insight into the cis-acting sequences and trans-acting factors either promote or inhibit nonsense-mediated mRNA decay (NMD). The specific aims are to: 1. Achieve insight into the mechanism by which the premature termination of GPx1 mRNA translation at the position of the Sec codon mediates cytoplamic mRNA decay. 2. Determine why premature termination of PHGPx mRNA translation at the position of the Sec codon fails to mediate a significant reduction in PHGPx mRNA abundance.