STRUCTURE/FUNCTION OF YEAST TRANSLATION FACTOR EIF4G

酵母翻译因子 EIF4G 的结构/功能

• 批准号: 6018984
• 负责人: ALAN B SACHS
• 金额: $ 26.24万
• 依托单位: UNIVERSITY OF CALIFORNIA BERKELEY
• 依托单位国家: 美国
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-09-01 至 2002-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6018984
• 关键词: RNA binding protein; Saccharomyces cerevisiae; adenosinetriphosphatase; enzyme activity; fungal genetics; gel mobility shift assay; genetic translation; immunoprecipitation; intermolecular interaction; molecular site; mutant; polyadenylate; protein binding; protein structure function; site directed mutagenesis; suppressor mutations; transcription factor; translation factor; transposon /insertion element; western blottings

The cap structure and the poly(A) tail on messenger RNA (mRNA) work together to stimulate translation initiation in yeast through their interaction with the translation initiation factor eIF4G. The cap binding protein eIF4E and the poly(A) tail binding protein Pab1p mediate the interaction of eIF4G with each of these mRNA structures. The RNA- activated ATPase eIF4A also interacts with eIF4G. This proposal outlines experiments whose goals are to understand how each of these proteins as well as RNA interacts with yeast eIF4G1 and with each other. Another goal is to understand whether eIF4A enzymatic activity or the association of the ribosome bound initiation factor eIF3 with eIF4G1 is stimulated when mRNA is circularized through the interaction of eIF4E and Pab1p with eIF4G1. A final goal of these experiments is to identify and then characterize other yeast proteins which either activate or repress the function of eIF4G1 and its associated proteins. Our results should help to elucidate the mechanisms by which translation initiation is stimulated and regulated in eucaryotes. This information will be invaluable in understanding alterations to the translational apparatus resulting from some types of cellular transformations or viral infections, and in developing assays and then compounds which will provide therapeutic options to correcting these alterations.

信使RNA的帽子结构和聚(A)尾巴的作用 共同刺激酵母中的翻译启动，通过它们的 与翻译起始因子eIF4G的相互作用。这顶帽子 结合蛋白eIF4E和聚(A)尾结合蛋白Pab1p介导 EIF4G与这些mRNA结构的相互作用。核糖核酸- 激活的ATPase eIF4A也与eIF4G相互作用。这项建议 概述了实验，其目标是了解每一个 蛋白质和RNA都能与酵母eIF4G1相互作用并相互作用。 另一个目标是了解eIF4A酶活性或 核糖体结合起始因子eIF3与eIF4G1的相关性 通过eIF4E的相互作用刺激mRNA循环 和带有eIF4G1的Pab1p。这些实验的最终目标是确定 然后对其他酵母蛋白进行表征，这些蛋白激活或 抑制eIF4G1及其相关蛋白的功能。我们的结果 应该有助于阐明翻译启动的机制 在真核细胞中受到刺激和调节。这一信息将是 在了解翻译设备的变化方面有非常宝贵的价值 由某些类型的细胞转化或病毒引起 感染，在发展的化验中，然后是化合物，这将 为纠正这些改变提供治疗选择。