CORE--TISSUE CULTURE FACILITY

核心——组织培养设施

• 批准号: 6203164
• 负责人: BARBARA L ZIFF
• 金额: $ 7.93万
• 依托单位: GEORGETOWN UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-07-30 至 2000-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6203164
• 关键词: biomedical facility; cancer registry /resource; cryopreservation; genetic screening; human tissue; neoplasm /cancer; tissue /cell culture

The primary functions of this shared resource are to manage the Cancer Cell Line Repository and to provide essential tissue culture support to cancer researchers. The facility provides shared tissue culture areas that are well equipped and maintained. Cancer cell lines are cultured and made available to investigators as either growing or frozen cell stocks. Frozen cell stocks are replenished as required. DNA fingerprinting of cell lines is used as quality control measure. The cancer cell line repository is managed through a complete inventory system. The individual cell lines of the repository are described in a computer database. A number of tissue culture related services are offered, including mycoplasma testing, helper virus rescue assays, processing of blood samples for genetic screening, charcoal stripping of calf serum, cell culture, cell freezing, DNA fingerprinting of cell lines, and growth curve analysis of new lots of both calf serum and fetal calf serum. The production of primary cell lines from normal breast tissue and from breast cancer primary or metastatic tumors is a developing service. The Tissue Culture Shared Resource is used by members of all of the programs of the CCSG, and it interacts with five of the other eight shared resources. Over 82% of the usage of the shared resource in the past year was by -reviewed projects of Cancer Center members.

此共享资源的主要功能是管理 癌细胞系资料库和提供必要的组织培养 对癌症研究人员的支持。该设施提供共享纸巾 设施齐全、维护完善的文化区。癌细胞 LINE被培养并作为以下任一种方式提供给调查人员 不断增长或冻结的细胞库存。冰冻的细胞库存得到补充 视需要而定。细胞系的DNA指纹图谱被用作质量 控制措施。癌细胞系资料库是通过 一个完整的库存系统。细胞的单个细胞系 在计算机数据库中描述了存储库。一些组织 提供与培养有关的服务，包括支原体检测， 辅助病毒救援化验，血液样本的处理 小牛血清的筛选、木炭剥离、细胞培养、细胞 冷冻、细胞系DNA指纹图谱和生长曲线 新一批小牛血清和胎牛血清的分析。这个 从正常乳腺组织和正常乳腺组织制备原代细胞系 乳腺癌、原发或转移性肿瘤是一项发展中的服务。 组织培养共享资源由所有 CCSG的项目，它与其他八个项目中的五个进行互动 共享资源。年内共享资源使用率超过82% 过去的一年是由癌症中心成员审查的项目。