CORE--BREAST CANCER CELL LINE RESOURCE

核心--乳腺癌细胞系资源

• 批准号: 6499560
• 负责人: BARBARA L ZIFF
• 金额: $ 13.53万
• 依托单位: GEORGETOWN UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-09-14 至 2003-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6499560
• 关键词: Mycoplasma; bioassay; biomedical facility; breast neoplasms; charcoal; chemical fingerprinting; clone cells; genetic transduction; growth media; helper virus; plasmids; tissue /cell culture; tissue resource /registry; transfection; transfection /expression vector

The primary functions of this core facility are to manage the breast cancer cell line repository and to provide essential tissue culture support to breast cancer researchers. The facility provides shared tissue culture areas that are well equipped and stocked with all of the necessary tissue culture reagents and supplies. Breast cancer cell lines are cultured and made available to investigators as either growing or frozen cell stocks. Frozen cell stocks are replenished as required. DNA fingerprinting of cell lines is used as a quality control measure. The breast cancer cell line repository is managed through a complete inventory system. The individual cell lines of the repository are described in a computer database that is part of the SPORE computer network. A number of tissue culture related services are offered, including mycoplasma testing, helper virus rescue assays, charcoal stripping of calf serum, cell culture, cell freezing, and growth curve analysis of new lots of both calf serum and fetal calf serum. The production of primary cell lines from normal breast tissue and from breast cancer primary or metastatic tumors is a developing service. The shared tissue culture areas are used by the investigators, trainees and technicians from Il LCRC breast cancer research laboratories who do not have their own individual tissue culture space. The breast cancer cell line repository is used by 13 breast cancer research laboratories. All of the breast cancer research laboratories regularly request mycoplasma testing. The helper virus rescue assay has been used by four breast cancer research laboratories. Charcoal stripped calf serum produced in the core facility is used by seven breast cancer research laboratories. The cell culture service has been used by eight breast cancer research laboratories.

这个核心设施的主要功能是管理乳房 癌细胞系库，并提供必要的组织培养 支持乳腺癌研究人员。该机构提供共享组织 文化区，设备齐全，并与所有的储备， 必要的组织培养试剂和用品。乳腺癌细胞系 培养并提供给研究人员， 冷冻细胞储备。根据需要补充冷冻细胞储备液。DNA 将细胞系的指纹鉴定用作质量控制措施。的 乳腺癌细胞系储存库是通过一个完整的 库存系统储存库的单个细胞系是 在作为SPORE计算机一部分的计算机数据库中描述 网络提供许多与组织培养相关的服务， 包括支原体检测、辅助病毒拯救试验、活性炭 小牛血清剥离、细胞培养、细胞冷冻和生长曲线 分析新批次的小牛血清和胎牛血清。的 从正常乳腺组织和从乳腺癌组织中产生原代细胞系 乳腺癌原发性或转移性肿瘤是一项发展中的服务。 研究者、受训者和研究人员使用共用的组织培养区。 Il LCRC乳腺癌研究实验室的技术人员， 没有自己的组织培养空间。乳腺癌 13个乳腺癌研究实验室使用细胞系储存库。 所有的乳腺癌研究实验室都定期要求 支原体检测辅助病毒拯救试验已被四个 乳腺癌研究实验室活性炭剥离小牛血清 在核心设施中生产的用于七个乳腺癌研究 laboratories.细胞培养服务已被八个乳腺癌患者使用， 癌症研究实验室