CORE--BREAST CANCER CELL LINE RESOURCE

核心--乳腺癌细胞系资源

• 批准号: 6663988
• 负责人: BARBARA L ZIFF
• 金额: $ 13.53万
• 依托单位: GEORGETOWN UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-09-16 至 2003-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6663988
• 关键词: Mycoplasma; bioassay; biomedical facility; breast neoplasms; charcoal; chemical fingerprinting; clone cells; genetic transduction; growth media; helper virus; plasmids; tissue /cell culture; tissue resource /registry; transfection; transfection /expression vector

The primary functions of this core facility are to manage the breast cancer cell line repository and to provide essential tissue culture support to breast cancer researchers. The facility provides shared tissue culture areas that are well equipped and stocked with all of the necessary tissue culture reagents and supplies. Breast cancer cell lines are cultured and made available to investigators as either growing or frozen cell stocks. Frozen cell stocks are replenished as required. DNA fingerprinting of cell lines is used as a quality control measure. The breast cancer cell line repository is managed through a complete inventory system. The individual cell lines of the repository are described in a computer database that is part of the SPORE computer network. A number of tissue culture related services are offered, including mycoplasma testing, helper virus rescue assays, charcoal stripping of calf serum, cell culture, cell freezing, and growth curve analysis of new lots of both calf serum and fetal calf serum. The production of primary cell lines from normal breast tissue and from breast cancer primary or metastatic tumors is a developing service. The shared tissue culture areas are used by the investigators, trainees and technicians from Il LCRC breast cancer research laboratories who do not have their own individual tissue culture space. The breast cancer cell line repository is used by 13 breast cancer research laboratories. All of the breast cancer research laboratories regularly request mycoplasma testing. The helper virus rescue assay has been used by four breast cancer research laboratories. Charcoal stripped calf serum produced in the core facility is used by seven breast cancer research laboratories. The cell culture service has been used by eight breast cancer research laboratories.

这一核心设施的主要功能是管理乳房 癌细胞系储存库和提供必要的组织培养 对乳腺癌研究人员的支持。该设施提供共享纸巾 设备齐全、储备齐全的文化区 必要的组织培养试剂和用品。乳腺癌细胞系 被培养并提供给调查人员，作为生长或 被冻结的细胞库存。冻结的细胞库存会根据需要进行补充。脱氧核糖核酸 细胞系的指纹图谱被用作质量控制措施。这个 乳腺癌细胞系存储库通过一个完整的 库存系统。储存库的单个细胞系是 在作为孢子计算机一部分的计算机数据库中描述 网络。提供了许多与组织培养相关的服务， 包括支原体检测，辅助病毒救援检测，木炭 小牛血清的剥离、细胞培养、细胞冷冻和生长曲线 新一批小牛血清和胎牛血清的分析。这个 从正常乳腺组织和正常乳腺组织制备原代细胞系 乳腺癌、原发或转移性肿瘤是一项发展中的服务。 共用组织培养区供研究人员、受训人员使用 和来自Il LCRC乳腺癌研究实验室的技术人员 没有自己的个人组织培养空间。乳腺癌 13个乳腺癌研究实验室使用细胞系资料库。 所有乳腺癌研究实验室定期要求 支原体检测。辅助病毒拯救试验已经被四个人使用 乳腺癌研究实验室。炭剥离小牛血清 在核心设施中生产的产品被七个乳腺癌研究机构使用 实验室。细胞培养服务已被八个乳房使用 癌症研究实验室。