CORE--BREAST CANCER CELL LINE RESOURCE

该核心设施的主要功能是管理乳房 癌细胞系储存库并提供必要的组织培养 支持乳腺癌研究人员。该设施提供共享组织 文化区设施齐全，备有所有 必要的组织培养试剂和用品。乳腺癌细胞系 被培养并提供给研究人员作为生长或 冷冻细胞库存。根据需要补充冷冻细胞库存。脱氧核糖核酸 细胞系指纹识别用作质量控制措施。的 乳腺癌细胞系存储库通过完整的管理 库存系统。存储库的各个细胞系是 在属于 SPORE 计算机一部分的计算机数据库中进行描述 网络。提供许多组织培养相关服务， 包括支原体检测、辅助病毒救援检测、木炭检测 小牛血清剥离、细胞培养、细胞冷冻和生长曲线 分析新批次的小牛血清和胎牛血清。的 从正常乳腺组织和从 乳腺癌原发性或转移性肿瘤是一项正在发展的服务。 共享的组织培养区域供研究人员、受训人员使用 以及来自 Il LCRC 乳腺癌研究实验室的技术人员 没有自己独立的组织培养空间。乳腺癌 细胞系存储库由 13 个乳腺癌研究实验室使用。 所有乳腺癌研究实验室都会定期要求 支原体检测。辅助病毒救援试验已被四位使用 乳腺癌研究实验室。木炭剥离小牛血清 核心设施生产的产品被七项乳腺癌研究使用 实验室。细胞培养服务已被八个乳房使用 癌症研究实验室。

The primary functions of this core facility are to manage the breast cancer cell line repository and to provide essential tissue culture support to breast cancer researchers. The facility provides shared tissue culture areas that are well equipped and stocked with all of the necessary tissue culture reagents and supplies. Breast cancer cell lines are cultured and made available to investigators as either growing or frozen cell stocks. Frozen cell stocks are replenished as required. DNA fingerprinting of cell lines is used as a quality control measure. The breast cancer cell line repository is managed through a complete inventory system. The individual cell lines of the repository are described in a computer database that is part of the SPORE computer network. A number of tissue culture related services are offered, including mycoplasma testing, helper virus rescue assays, charcoal stripping of calf serum, cell culture, cell freezing, and growth curve analysis of new lots of both calf serum and fetal calf serum. The production of primary cell lines from normal breast tissue and from breast cancer primary or metastatic tumors is a developing service. The shared tissue culture areas are used by the investigators, trainees and technicians from Il LCRC breast cancer research laboratories who do not have their own individual tissue culture space. The breast cancer cell line repository is used by 13 breast cancer research laboratories. All of the breast cancer research laboratories regularly request mycoplasma testing. The helper virus rescue assay has been used by four breast cancer research laboratories. Charcoal stripped calf serum produced in the core facility is used by seven breast cancer research laboratories. The cell culture service has been used by eight breast cancer research laboratories.