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TISSUE DIELECTRIC CHANGES DUE TO HYPERTHERMIA

高温引起的组织介电变化

基本信息

批准号：
2894930
负责人：
DONALD A MC RAE
金额：
$ 17.8万
依托单位：
GEORGETOWN UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
1993
资助国家：
美国
起止时间：
1993-05-05 至 2001-07-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/2894930
关键词：
apoptosis athymic mouse cell line electrical impedance electron microscopy histopathology human tissue hyperthermia method development necrosis neoplasm /cancer diagnosis neoplastic process noninvasive diagnosis verapamil xenotransplantation

项目摘要

DESCRIPTION: Cancer therapies of all forms, radiation, cytotoxic drugs, hyperthermia, etc., have one goal in common. That goal is to eradicate cancer cells. Therefore, when therapies are being tested in the laboratory or applied in the clinic, the ability to which they eradicate the cancer cells needs to be evaluated. Typically, endpoints such as tumor-growth-delay or tumor regression are used, or if intermediate information is desired, invasive tissue samples must be taken. Recently, it has been determined that cells predominantly respond to all forms of treatment of deleterious stimuli in two ways; via programmed cell death (apoptosis) and/or via necrosis. The aim of this proposal is to test the capability of Electrical Impedance Spectroscopy (EIS) to detect and identify these two forms of cell response via non-invasive, in vivo measurements in solid human xenografts grown in nude mice. Treatment with hyperthermia, with and without verapamil, will be used to induce the different forms of cell response. Then electrical impedance measurements of the tumors will be made during the period between application of the treatment and manifestation of tumor-growth-delay. These EIS measurements will aim to monitor the real-time cellular-level response of the tumor during this period. The work will test the hypotheses: 1. It is possible to make long-term, reliable, repeatable, non-invasive, in vivo Electrical Impedance Spectroscopy (EIS) measurements of the electrical properties of tissues. 2. EIS is capable of detecting and characterizing long-term, hyperthermia-induced, necrotic response sequences during their occurrence, and of discriminating between different characteristics in the necrotic responses caused by different treatments which result in different tumor-growth-delays. 3. EIS is capable of detecting and characterizing the apoptotic cell response sequence within a solid tumor with high apoptotic index, using non-invasive, in vivo measurements. Progress and preliminary data demonstrate the feasibility of measuring cellular-level change with EIS.

描述：各种形式的癌症治疗，放射，细胞毒性药物，体温过高等，都有一个共同的目标我们的目标是根除癌细胞因此，当在实验室测试疗法时或应用于临床，他们根除癌症的能力细胞需要评估。通常，端点，例如使用肿瘤生长延迟或肿瘤消退，或者如果是中间状态，如果需要信息，则必须获取侵入性组织样本。近日已经确定细胞主要对所有形式的以两种方式治疗有害刺激;通过程序性细胞死亡（凋亡）和/或通过坏死。本提案的目的是测试电阻抗的能力光谱（EIS）检测和识别这两种形式的细胞反应通过非侵入性的，在体内测量生长在裸鼠使用或不使用维拉帕米的高温治疗，用于诱导不同形式的细胞反应。然后是电气肿瘤的阻抗测量将在肿瘤生长延迟的治疗和表现的应用。这些 EIS测量的目的是监测实时蜂窝级响应肿瘤在此期间本研究将验证以下假设：1. 有可能实现长期、可靠、可重复、无创、体内电阻抗组织电特性的光谱（EIS）测量。 2. EIS能够检测和表征长期，高血压诱导的坏死反应序列在其发生期间，以及区分坏死组织中不同特征的能力不同的治疗引起的反应，肿瘤生长延迟。 3. EIS能够检测和表征具有高凋亡的实体瘤内的凋亡细胞反应序列指数，使用非侵入性，在体内测量。进展及初步数据证明了测量细胞水平变化的可行性 EIS。