BETA 6 INTEGRIN IN THE MACULA DENSA

致密斑中的 Beta 6 整合素

• 批准号: 2904986
• 负责人: LOIS J AREND
• 金额: $ 12.28万
• 依托单位: UNIVERSITY OF ROCHESTER
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-09-20 至 2000-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2904986
• 关键词: confocal scanning microscopy; developmental genetics; embryo /fetus tissue /cell culture; flow cytometry; gene expression; histogenesis; immunologic assay /test; immunoprecipitation; in situ hybridization; integrins; laboratory mouse; molecular cloning; northern blottings; nucleic acid sequence; polymerase chain reaction; protein kinase; protein sequence; protein structure; renal tubule

The macula densa is an important regulatory portion of the nephron. Located where the thick ascending limb of Henle's loop contacts the glomerulus, the macula densa plays a role in transmitting information about tubular fluid composition to the glomerulus, and afferent and efferent arterioles, thereby effecting changes in renal blood flow, renin release, and glomerular filtration rate. A rather rare integrin, the beta6 subunit, is expressed in the macular densa, particularly during development of the kidney. Since the macula densa has an important role in regulating function of the kidney, the study of a protein such as the beta6 integrin that is highly expressed in this region may provide clues to the development and regulation of the macula densa. To investigate the hypothesis that beta6 integrin is important in macula densa development and functin, the complete cDNA sequence of the mouse homologue of beta6 integrin will be determined and antibodies to the beta6 integrin extracellular and cytoplasmic, domains developed. These antibodies will be used to examine the expression pattern of beta6 integrin in the developing kidney, to isolate macula densa cells, and to evaluate the functional role of beta6 integrin in the macula densa.

黄斑丁萨是重要的调节部分 肾单位。 位于Henle循环的厚边缘 接触肾小球，黄斑丹萨在 将有关管状流体组成的信息传输到 肾小球以及传入和传染性动脉，从而产生 肾脏血流，肾素释放和肾小球过滤的变化 速度。 一个相当罕见的整合素，beta6亚基，在 黄斑丁萨，特别是在肾脏发育过程中。 由于黄斑densa在调节中具有重要作用 肾脏的功能，诸如β6之类的蛋白质的研究 在该区域高度表达的整合素可能会为 黄斑丹森的发展和调节。 研究β6整联蛋白在 黄斑densa的开发和功能，完整的cDNA β6整合素的小鼠同源素的顺序将是 确定的和抗Beta6整合素外细胞外抗体和抗体 细胞质，结构域发展。 这些抗体将用于 在发育中检查β6整合素的表达模式 肾脏，分离黄斑丁莎细胞，并评估功能 β6整联蛋白在黄斑densa中的作用。

项目成果

Three-dimensional imaging of embryonic mouse kidney by two-photon microscopy.

双光子显微镜对小鼠胚胎肾脏的三维成像。

• DOI: 10.1016/s0002-9440(10)63943-0
• 发表时间: 2001
• 期刊: The American journal of pathology
• 作者: Phillips,CL;Arend,LJ;Filson,AJ;Kojetin,DJ;Clendenon,JL;Fang,S;Dunn,KW
• 通讯作者: Dunn,KW

Dynamic regulation of sphingosine-1-phosphate homeostasis during development of mouse metanephric kidney.

• DOI: 10.1152/ajprenal.90232.2008
• 发表时间: 2009-03
• 期刊: American journal of physiology. Renal physiology
• 作者: R. Jason Kirby;Ying Jin;Jian-Lin Fu;Jimena Cubillos;D. Swertfeger;Lois J. Arend