CLINICAL EXPERIMENT OF HELICOBACTER PYLORI TRANSMISSION

描述(取自应用程序) 幽门螺杆菌感染是最常见的细菌感染之一。 在世界范围内造成严重的发病率和死亡率。然而，到了 日期，我们不知道有机体是如何传播的。虽然大多数数据 来自美国、欧洲和澳大利亚的直接个人对个人支持 传播，这发生的方式是未知的。我们假设 通过直接接触呕吐物或喷雾剂进行胃口传播 呕吐物是最有可能的传播途径。我们进一步 假设胃酸过少可能促进活菌的排泄 有机体。这项研究的具体目的是评估是否可行 呕吐物中存在幽门螺杆菌细菌，期间采集的空气样本 呕吐、唾液和/或粪便。为了做到这一点，我们打算进行一项临床 在30名幽门螺杆菌感染的志愿者中进行实验。在确认H. 幽门螺杆菌感染的血清学和13C尿素呼气试验，受试者将 提供唾液和粪便样本用于幽门螺杆菌检测。其中的一半 受试者将接受为期两周的西咪替丁治疗，而其他患者将接受为期两周的治疗 不接受组胺拮抗剂。在这段时间结束时，所有的受试者 将被斯坦福大学的临床研究部接纳，在那里进行宣泄 而呕吐将被顺序地诱导。大便、呕吐物、唾液和空气 样本将通过四种方法进行幽门螺杆菌检测：常规培养， 免疫磁分离后培养，免疫磁分离 然后是聚合酶链式反应和常规聚合酶链式反应(仅限空气过滤器样品)。通过对H。 将对呕吐物、空气、粪便和唾液中的幽门螺杆菌进行比较。 目前，与幽门螺杆菌感染有关的健康干预措施包括 治疗幽门螺杆菌相关疾病，而不是预防感染。 预防性疫苗的可获得性还需要几年到几十年的时间。 了解幽门螺杆菌的传播途径(S)是一个关键的开始 疾病预防的点，并将实现更有针对性的目标 干预策略。

DESCRIPTION (taken from the application) Helicobacter pylori infection is among the most common bacterial infections of man, causing significant morbidity and mortality worldwide. Yet, to date, we do not understand how the organism is transmitted. While most data from the United States, Europe and Australia support direct person-to-person spread, the manner in which this occurs is unknown. We hypothesize that gastric-oral transmission via direct contact with vomitus or aerosolization of vomitus is the most probable means of transmission. We further hypothesize that gastric hypochlorhydria may foster excretion of viable organisms. The specific aims of this study are to evaluate whether viable H. pylori organisms are present in vomitus, air samples obtained during vomiting, saliva and/or feces. To do this, we intend to conduct a clinical experiment in 30 H. pylori infected volunteers. After confirmation of H. pylori infection using serology and 13C urea breath test, subjects will provide saliva and a stool sample for H. pylori testing. One half of these subjects will then be treated with cimetidine for two weeks while the others receive no histamine antagonist. At the end of this period, all subjects will be admitted to the Clinical Research Unit at Stanford where catharsis and emesis will be sequentially induced. Stool, vomit, saliva and air samples will be tested for H. pylori four ways: routine culture, immunomagnetic separation followed by culture, immunomagnetic separation followed by PCR and routine PCR (air filter samples only). The yield of H. pylori from vomitus, air, feces and saliva will be compared. Currently, health interventions related to H. pylori infection involve treatment of H. pylori-related diseases rather than prevention of infection. Availability of prophylactic vaccines remains years to decades away. Understanding the route(s) of H. pylori transmission is a critical starting point for disease prevention and will enable more directed targeting of intervention strategies.