ANALYSIS OF PAROTID GLAND 4B-GALACTOSYLTRANSFERASE

慢性注射β-肾上腺素能激动剂，特异性 提高细胞内cAMP水平和某些饮食变化，产生 大鼠腮腺细胞肥大和增生。 此外，高尔基体的特定活动也有所增加 标记酶4β-半乳糖基转移酶。这种活动的变化是 伴随着这种酶在亚细胞内定位的改变 质膜。上调监管和浮出水面的重要性 局部化在实验中变得明显，在这些实验中， 与这种酶相互作用被证明严重抑制腮腺 在体外或体外发生的细胞肥大和增殖 活着。大量报道显示细胞表面半乳糖的改变 T‘酶活性可能参与了细胞的黏附、分化和 肿瘤发生。转移酶调控的分子研究， 对于理解腮腺和其他组织的生长控制至关重要 纸巾。 我们对唾液腺发育和 分化是由生理和组织学指导的 观察。我的实验室提供了新的见解 分离的腺泡细胞生长的生化和分子基础 4β-半乳糖T‘酶的基因克隆。我们的长期目标是了解 Gal T‘ase基因的复杂调控及其对细胞的调节作用 成长。刚果民盟将允许我有时间进行几项活动，这些活动 会促进我的研究发展。在颁奖过程中， 我们建议发起与其他唾液腺的合作 在细胞生物学方面有专长的研究人员。我们的兴趣之一是 半乳糖T‘酶在发育过程中与细胞外相互作用中的作用 基质及其对细胞迁移的贡献。RCDA还将 请允许我花时间扩大我与 牙医学院。对人类病理学中相关性的追求 涉及口腔组织的增生症将需要闭合 与临床和研究人员合作。这些经历 将是非常有价值的，并可以在我未来的研究中应用 腮腺生长发育。

Chronic injections of beta-adrenergic agonist, agents that specifically raise intracellular cAMP levels and certain dietary changes, produce cell hypertrophy and hyperplasia in the rat parotid gland. Additionally, there is an increase in specific activity of the Golgi marker enzyme 4beta-galactosyltransferase. This change in activity is accompanied by an altered subcellular localization of this enzyme to the plasma membrane. The importance of up-regulation and surface localization became apparent in experiments in which agents that interact with this enzyme were shown to severely inhibit parotid gland cell hypertrophy and hyperplasia from taking place either in vitro or in vivo. Numerous reports have shown the alteration of cell surface Gal T'ase activity might be involved in cell adhesion, differentiation and tumorogenesis. Molecular studies on the regulation of the transferase, are crucial to understanding growth control in parotid and other tissues. Our basic understanding of salivary gland development and differentiation are guided by physiological and histological observations. My laboratory has provided new insights into the biochemical and molecular basis of acinar cell growth by having isolated a cDNA clone to 4beta-Gal T'ase. Our long-term goal is to understand the complex regulation of the Gal T'ase gene and how it mediates cell growth. The RCDA would allow me time to pursue several activities which would enhance my research development. During the course of the award, we propose to initiate collaborations with other salivary gland researchers with expertise in cell biology. One of our interests is the role Gal T'ase plays in developmental interactions with extracellular matrix and its contribution to cell migration. The RCDA would also allow me time for expanding my interactions with the faculty in the College of Dentistry. The pursuit of correlations in human pathologies of the oral cavity involving tissue hyperplasia will require close cooperation with the clinical and research faculty. These experiences would be extremely valuable and could be applied in my future studies of parotid gland growth and development.