ION CURRENTS IN FERTILIZATION AND EARLY DEVELOPMENT

最初由受精引发的事件涉及离子变化。那里 也是质膜离子渗透性的立即变化 这是细胞内离子浓度的巨大变化。这些离子 事件必须是启动发展的重要步骤，因为 对鸡蛋离子环境的简单操作通常会激活 孤雌生殖的发育。这项研究将研究两者的作用 细胞内离子电流和细胞内离子浓度的变化 两种脊椎动物卵的激活和卵质分离 青蛙、非洲爪哇、大米等鱼，使用了一些新的 技术：将使用振动探头技术来进行电气检测 受精所触发的胞外离子电流。Ca++、H+、Na+ 而氯离子浓度将使用细胞内特定的离子来研究 微电极。之后发生的第一批发展事件之一 受精是一种动态的细胞质再分配，称为“卵质受精”。 由于有证据表明，这一过程也受到了影响 通过离子流和离子梯度，研究了这些早期离子的作用 种族隔离的变化将是这项研究的自然延伸。这个 质膜驱动的稳定的跨细胞离子电流 在这种分离过程中的卵子将被绘制成地图，离子成分 将会被确认。这项工作的一个主要目标是确定这些 洋流在隔离机制中扮演着偶然的角色。

The events first triggered by fertilization involve ionic changes. There is an immediate change in the plasma membrane's ion permeability, as well as a dramatic change in intracellular ion concentrations. These ionic events must be important steps in the activation of development because simple manipulations of the egg's ionc environment will often activate development parthenogenetically. This research will study the role of both transcellular ion currents and intracellular ion concentration changes in activation and ooplasmic segregation in two vertebrate eggs, that of the frog, Xenopus laevis, and the fish Oryzias latipes, using a number of new techniques: Avibrating probe technique will be used to electrically detect the extracellular ion currents triggered by fertilization. Ca++, H+, Na+, and Cl-ion concentrations will be studied using intracellular ion-specific microelectrodes. One of the first developmental events occurring after fertilization is a dynamic cytoplasmic redistribution termed "ooplasmic segregation." Since there is evidence that this process is also influenced by ion currents and gradients, a study of the roles of these early ionic changes in segregation would be a natural extension of this research. The steady transcellular ion currents which the plasma membrane drives through the egg during this segregation will be mapped and the ionic components will be identified. A major goal of this work is to determine if these currents play a casual role in the segregation mechanism.