EFFECTS OF ZINC OXIDE WELDING FUME INHALATION

类似流感的发烧、肌肉酸痛和呼吸主诉的综合症称为 “金属烟雾热”与吸入氧化锌烟雾有关。 尽管接触过这种常见疾病的人和 在职业卫生保健提供者中，对 其致病机理--氧化锌烟雾的剂量效应 关系，以及疾病的预防和治疗。我 假设氧化锌烟雾导致人体内细胞因子的释放 这些细胞因子起内源性热原和趋化作用 炎症反应的因素。我建议检验一下这一假设 并为观察到的影响生成详细的剂量响应数据 镀锌低碳钢电弧焊研究课题 在环境曝光室里。我会测量肺功能， 包括对乙酰甲胆碱的支气管反应性，基线，1小时 以及暴露后20小时。我会的。进行支气管肺泡灌洗 (BAL)在暴露后22小时，并在2至4个月之间再次使用。我 将分析BAL的细胞总数和差异，并进行测量 用双抗体夹心法测定白细胞介素1(IL-1)、肿瘤坏死因子(TNF)。这就做 同时检测BAL细胞中IL-1mRNA的表达。我会把这些联系起来 氧化锌暴露数据的生理和细胞效应 通过个人呼吸带监测和血锌采集 级别。锌介导的金属烟雾热是一组职业性疾病之一 疾病，包括因吸入聚合物和有机粉尘而引起的综合征， 暗示了共同的病理生理机制。这项研究针对的是 肺和全身综合征是中国的一个重要临床问题 职业医学。确定与细胞因子的关联，同时 描绘氧化锌烟雾的剂量反应将有助于 对金属烟雾热等疾病病因及预防的认识 类似的职业病。

The flu-like syndrome of fever, myalgia, and respiratory complaints called "metal fume fever" is associated with inhalation of zinc oxide fume. Despite familiarity with this common condition among those exposed and among occupational health care providers, little is known about the mechanisms of its causation, the zinc oxide fume dose response relationship, and the prevention and treatment of the disorder. I hypothesize that zinc oxide fume causes the release of cytokines in the lung and that these cytokines act as endogenous pyrogens and chemotactic factors for an inflammatory response. I propose to test this hypothesis and to generate detailed dose response data for the effects observed by studying subjects performing electric arc welding on galvanized mild steel in an environmental exposure chamber. I will measure pulmonary function, including bronchial responsiveness to methacholine, at baseline, 1 hour and 20 hours after exposure. I will.carry out bronchoalveolar lavage (BAL) at 22 hours and once again between 2 to 4 months after exposure. I will analyze BAL for total cell count and differential and measure interleukin 1 (IL-1) and tumor necrosis factor (TNF) by ELISA. I will also measure mRNA for IL-1 in the BAL cells. I will correlate these physiologic and cellular effects with exposure data for zinc oxide collected by personal breathing zone monitoring and with blood zinc levels. Zinc mediated metal fume fever is one of a group of occupational disorders, including syndromes due to polymer and organic dust inhalation, suggesting common pathophysiologic mechanisms. This study addresses a pulmonary and systemic syndrome that is an important clinical problem in occupational medicine. Identifying an association with cytokines while delineating the dose response to zinc oxide fume will contribute to an understanding of the causes and prevention of metal fume fever and other similar occupational syndromes.