LINEAL ORIGIN OF NEURON GROUPS

候选人将从事研究事业，重点是胚胎 克隆相关神经元的分化和差异的方式 在神经元形态学、生理学和生物化学（递质类型）方面， 不同的解剖学和生理学连接， 成熟的中枢神经系统的回路。建议的实验将 解决了我们所拥有的刻板神经元群的直系起源问题， 最近在蝗虫Schistocerca的成年神经节中描述 美洲，和不同的表型性状的表达， 在这些群体中单独识别的神经元。的重要前提 这些目标的实现是为候选人发展熟练 在许多方法中，主要是那些与谱系分析有关的方法， 在胚胎中。这些方法的专业知识可以在埃默里大学部分学习 大学和部分机构的同事。一个研究 职业发展奖将大大促进这一进程， 有更多机会从事研究活动的候选人（85%） 通过减少对教学和大学服务的承诺。我们 具体的目标包括研究一组神经元， 从单个神经母细胞胚胎产生。该组织约有20名成员， 不同表型的神经元，包括多巴胺能运动神经元， 节段间的中间神经元可能是GABA能的，是一种多分支的传出神经元， 可能与其他昆虫的肽能神经元同源， 不确定数量的局部中间神经元。我们将描述 和电生理特性的组中的神经元， 成年人，这样我们就可以识别每个人。我们将确定 哪些属性由组的所有成员共享，哪些属性 对某些人来说独一无二。然后我们将开始对胚胎的研究， 假设神经元是克隆相关的。为了追溯血统， 使用常规技术进行染料的细胞内注射， 用改变的核苷BUdR标记的新开发技术。 用染料、BUdR和其他抗体的标记将在一个紧密的环境中进行。 时间序列，以确定不同的起源的相对时间 神经元和获得不同的表型性状。

The candidate will pursue a research career that focuses on the embryonic differentiation of clonally-related neurons and on the way that differences in neuronal morphology, physiology and biochemistry (transmitter type) lead to differing anatomical and physiological connections in the neuronal circuitry of the mature central nervous system. Experiments proposed will address the lineal origins of stereotyped neuron groups which we have recently described in the adult ganglia of the grasshopper Schistocerca americana, and the expression of differing phenotypic traits in individually-identified neurons in these groups. A major prerequisite for the realization of these goals is for the candidate to develop proficiency in a number of methodologies, primarily those related to lineage analysis in the embryo. Expertise in these methods can be learned partially at Emory University and partially at the institutions of colleagues. A Research Career Development Award would greatly facilitate this process by providing the candidate with greater opportunity for research activities (85%) through reduction in commitments to teaching and university service. Our specific aims involve the study of one group of neurons that we suggest arise embryonically from a single neuroblast. The group contains some 20 neurons of diverse phenotype, including glutaminergic motor neurons, intersegmental interneurons that may be GABA-ergic, a multibranced efferent that may be homologous to peptidergic neurons from other insects and an indeterminate number of local interneurons. We will describe the anatomical and electrophysiological properties of the neurons in the group in the adult, so that we can identify each as an individual. We will determine which properties are shared by all members of the group and which are unique to some. We will then begin work on the embryo, to test our hypothesis that the neurons are clonally related. To trace lineage we will use conventional techniques for the intracellular injection of dye and newly-developed techniques for labeling with an altered nucleoside BUdR. The labeling with dyes, BUdR and other antibodies will be done in a closely timed series to determine the relative time of origin of the various neurons and of acquisition of differing phenotypic traits.