MICROVASCULAR FLUID AND SOLUTE EXCHANGE

此RCDA应用程序的目的是为我的 作为独立调查员在以下领域开展工作 微循环。我的重点是研究…… 流体和溶质在毛细血管和血管内的运动 间隙空间。在许多病理情况下，存在一种不平衡 在血管和血管之间的流体和溶质的分配 血管外空间。通过微血管系统的液体交换是 部分受间质静水和肿胀的控制 压力。间质肿胀压力由 血管外血浆蛋白在间质凝胶中的分布 基质，由胶原蛋白和糖胺聚糖组成。因此，我的目标是 血浆蛋白在间质中的转运和分布研究 凝胶基质，由胶原蛋白和糖胺聚糖组成。因此，我的目标是 目的：研究血浆中蛋白质的转运和分布。 在不同类型的水肿中的间质，并将数据与 提出关于控制体内液体平衡的概念。我们的 初步研究表明，血浆蛋白通过 生理盐水扩张后的间质基质不同于 静脉压升高或毛细血管壁增厚后 对血浆蛋白的通透性。我建议检验一下这个假设 血浆蛋白通过游离液从血浆输送到淋巴。 静脉充血或内皮细胞改变后的通道 渗透性，并在盐水膨胀或 慢性血浆透出症。在麻醉的兔子身上，我们将确定 血浆中示踪剂白蛋白和免疫球蛋白的初始平衡速率 淋巴。我将研究兔子后爪的皮肤和骨骼肌。 我会比较示踪白蛋白在血浆和血浆中的平衡率 淋巴是血浆和间质之间的淋巴。我也会测试一下 除了位阻外，还有静电排斥的假说 从间质凝胶的部分中排除白蛋白。这些结果 将提供有关间质基质在组织中的作用的信息 体液平衡以及对水肿的病因的洞察 队形。这个项目也将成为我研究工作的重点 在接下来的5年里，随着我作为一名职业生理学家的职业发展 微循环领域的独立科学家。

The intent of this RCDA application is to provide support for my development as an independent investigator in the area of microcirculation. My emphasis is directed towards the study of transvascular movement of fluid and solutes across capillaries and the interstitial space. In many pathological conditions, there is an imbalance in the partition of fluid and solutes between the vascular and extravascular spaces. Fluid exchange across the microvasculature is partially controlled by the interstitial hydrostatic and oncotic pressures. The interstitial oncotic pressure is determined by the distribution of extravascular plasma proteins in the interstitial gel matrix, composed of collagen and glycosaminoglycans. Thus, my goal is to study the transport and distribution of plasma proteins in the interstitial gel matrix, composed of collagen and glycosaminoglycans. Thus, my goal is to study the transport and distribution of plasma proteins in the interstitium during different types of edema and relate the data to the present concepts on the control of fluid balance in the body. Our preliminary studies indicate that the transport of plasma proteins through the interstitial matrix following saline expansion is different than that following increased venous pressure or increased capillary wall permeability to plasma proteins. I propose to test the hypothesis that plasma proteins are carried from plasma to lymph through free fluid channels following venous congestion or alterations in endothelial permeability, and through the gel matrix following saline expansion or chronic plasmaphoresis. In anesthetized rabbits we will determine the initial equilibration rates for tracer albumin and IgG from plasma to lymph. I will study both skin and skeletal muscle in the rabbit hindpaw. I will compare the equilibration rate for tracer albumin between plasma and lymph tothat between plasma and the interstitial space. I will also test the hypothesis that there is electrostatic exclusion, in addition to steric exclusion, of albumin from portions o the interstitial gel. These results will provide information on the role of the interstitial matrix in tissue fluid balance as well as provide insight into the etiology of edema formation. This project will also form a focus for my research efforts over the next 5 years as I develop my career as a professional physiologist and independent scientist in the field of microcirculation.