CAPILLARY AND INTERSTITIAL FLUID AND SOLUTE EXCHANGE

在许多病理情况下，存在着不平衡的划分 血管和血管外间隙之间的液体和溶质。变化 在水合的间质凝胶基质会影响 血浆蛋白在这两个隔室之间的分布及其变化 流体分布。拟议研究的总体目标是 确定血浆蛋白在体内分布和转运的变化 皮肤和骨骼肌的间质，并将数据与 提出关于控制体内液体平衡的概念。我的前辈 研究表明，血浆蛋白在血管外的分布 生理盐水扩张或血浆置换后的皮肤和骨骼肌 不同于以下升高的静脉压或升高 毛细管壁渗透性。这可能会对 血管和间质之间的液体平衡 全身间质液体的一部分由皮肤和 骨骼肌。我建议测试一下血浆蛋白是 通过体内的自由液体通道直接从血浆输送到淋巴 静脉充血或内皮细胞改变后的间质 渗透性和通过以下间质凝胶基质 血浆置换。我将在皮肤和骨骼肌中研究这一点 麻醉兔的后腿。我会比较平衡率 用于示踪间质中的白蛋白到淋巴中的白蛋白 无论白蛋白是通过自由流体通道还是通过 凝胶基质。我还将确定不同形式的浮肿的影响 关于白蛋白的静电排斥。这将通过研究 示踪白蛋白的血管外转运和分布，它具有 改变了装药量，但没有改变大小。更多的研究将比较这些影响。 血管外高蛋白血症对低蛋白血症的影响 液体和血浆蛋白在皮肤和骨骼肌中的分布。 这些结果将提供有关间质作用的信息。 组织液平衡中的基质以及对病因的洞察 浮肿形成的可能性。

In many pathological conditions, there is an imbalance in the partition of fluid and solutes between the vascular and extravascular spaces. Changes in the hydration of the interstitial gel matrix will effect the distribution of plasma proteins between these two compartments and alter the fluid distribution. The general aim of the proposed study is to determine changes in the distribution and transport of plasma proteins in the interstitium of skin and skeletal muscle and relate the data to the present concepts on the control of fluid balance in the body. My previous studies indicate that the extravascular distribution of plasma proteins in skin and skeletal muscle following saline expansion or plasmapheresis is different from that following increased venous pressure or increased capillary wall permeability. This may have important consequences on the fluid balance between the vascular and interstitial spaces since the major fraction of the total body interstitial fluid is represented by skin and skeletal muscle. I propose to test the hypothesis that plasma proteins are carried directly from plasma to lymph through free fluid channels within the interstitium following venous congestion or altered endothelial permeability and through the interstitial gel matrix following plasmapheresis. I will study this in both skin and skeletal muscle in the hind leg of anesthetized rabbits. I will compare the equilibration rate for tracer albumin in the interstitium to that in lymph to determine whether albumin is transported through free fluid channels or through the gel matrix. I will also determine the effects of different forms of edema on electrostatic exclusion of albumin. This will be done by studying the extravascular transport and distribution of tracer albumin, which has an altered charge but not size. Additional studies will compare the effects of hyperproteinemia to those of hypoproteinemia on the extravascular distribution of fluid and plasma proteins in skin and skeletal muscle. These results will provide information on the role of the interstitial matrix in tissue fluid balance as well as provide insight into the etiology of edema formation.