CAPILLARY & INTERSTITIAL FLUID & SOLUTE EXCHANGE

血浆蛋白的血管外分布受以下因素的影响： 血管屏障的选择性和相互关联的 胶原蛋白和糖胺聚糖的基质 interstitium. 在我们目前的工作中，我们发现， 血管外白蛋白和IgG的质量， 水肿不平行于淋巴蛋白浓度的变化 在皮肤和骨骼肌中。 我们的假设是血浆 蛋白质分布在两个隔室：一个非限制性的 一种与淋巴液自由交换， 其在空间上和静电上限制运动， 血浆蛋白分布。 有一种跨血管蛋白 流入两个隔室;然而，蛋白质流入 限制性隔室主要是扩散，而 主要是对流性的 的 经血管转运进入限制性室取决于 主要是血浆蛋白的电荷，而进入 淋巴区取决于大小。 的影响 蛋白质转运的限制性隔室更多 在皮肤中占主导地位，其胶原蛋白含量高， 肌肉. 示踪剂白蛋白和IgG的比活性将是 在淋巴和组织中测量， 恒定的血浆活性，以确定相对大小， 两个隔间的周转率。 皮肤数据将 与骨骼肌的数据进行比较，以确定 隔室行为在皮肤中更占优势。 数据 将血浆置换后获得的数据与 在静脉压升高后获得，以检验假设 降低等离子体的初始压力， 而增加微血管压力会影响 非限制性隔间。 单体的平衡 和二聚体白蛋白的不同等电点进行研究 以确定电荷对血管外 血浆蛋白分布。 初始组织摄取率 不同等电点的单体和二聚体白蛋白 将与稳态淋巴细胞-血浆 浓度比，以确定 电荷、微血管选择性和淋巴浓度。 这些结果将提供有关的作用， 间质基质与组织液平衡的关系 淋巴蛋白浓度和微血管 选择性

The extravascular distribution of plasma proteins is effected by both the selectivity of the vascular barrier and an interlinked matrix of collagen and glycosaminoglycans within the interstitium. In our present work, we found that changes in the extravascular mass of albumin and IgG following interstitial edema did not parallel changes in the lymph protein concentration in both skin and skeletal muscle. Our hypothesis is that plasma proteins distribute within two compartments: a non-restrictive one which exchanges freely with lymph and a restrictive one which sterically and electrostatically restrict the movement and distribution of plasma proteins. There is transvascular protein flux into both compartments; however, the protein flux into the restrictive compartment is predominately diffusion, while that into the lymph compartment is predominately convective. The transvascular transport into the restrictive compartment depends mainly upon the charge of the plasma protein, while that into the lymph compartment depends upon size. The effects of the restrictive compartment on protein transport are more predominate in skin, with its high collagen content, than skeletal muscle. The specific activity of tracer albumin and IgG will be measured in lymph and tissue at different times following a constant plasma activity to determine the relative sizes and turnover rates of the two compartments. Data from skin will be compared to data from skeletal muscle to determine if the two compartment behavior is more predominant in skin. Data obtained following plasmapheresis will be compared to data obtained after increased venous pressure to test the hypothesis that decreasing plasma oncotic pressure effects the restrictive compartment while increasing microvascular pressure effects the non-restrictive compartment. The equilibration of monomeric and dimeric albumin of different isoelectric points will be studied to determine the importance of charge on the extravascular distribution of plasma proteins. The initial tissue uptake rate of monomeric and dimeric albumins of different isoelectric points will be compared to the steady-state lymph-to-plasma concentration ratios to determine the relationship between charge, microvascular selectivity, and lymph concentrations. These results will provide information on the role of the interstitial matrix in tissue fluid balance and the relationship between lymph protein concentrations and microvascular selectivity.