INSULIN-SPECIFIC ACTIVATION OF IMMUNE T CELLS

免疫 T 细胞的胰岛素特异性激活

• 批准号: 3080438
• 负责人: DAVID N SHAPIRO
• 金额: $ 3.84万
• 依托单位: UNIVERSITY OF MICHIGAN AT ANN ARBOR
• 依托单位国家: 美国
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-07-01 至 1988-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3080438
• 关键词: antireceptor antibody; autoimmune disorder; autoradiography; calcium channel blockers; calcium metabolism; clone cells; cyclic AMP; cyclic GMP; helper T lymphocyte; histocompatibility antigens; hybridomas; immunochemistry; immunoregulation; interleukin 2; leukocyte activation /transformation; macrophage; monoclonal antibody; phosphorylation; protein kinase; radiotracer

The objectives of this project are to determine the structure and function of the T-cell immune recptor for insulin and to ascertain how its modulation affects antigen specific T-cell activation. These objectives will be accomplished by using established T-cell clones and newly generated T-cell hybridomas reactive with bovine insulin to prepare antibodies that specifically recognize the T-cell immune receptor. These anti-receptor reagents will then be used to invessigate and modulate the biochemical and physiological events which accompany antigen-specific activation of T cells and culminate in final T-cell effector function. The biochemical and functional characterization of the T-cell receptor and the intracellular events it modulates will be critical to our understanding of the factors controlling the antigen-specific response of this immunoregulatory cell. Aberrant regulation of the immune system has been implicated in the etiology of Type I diabetes mellitus and such therapeutic problems as insulin "allergy" and the development of insulin antibodies. Delineation of the mechanism and consequences of immune T-cell recognition of insulin will allow strategies for specific immunotherapy in diabetes.

本项目的目标是确定 胰岛素的T细胞免疫受体，并确定其如何 调节影响抗原特异性T细胞活化。 这些目标 将通过使用已建立的T细胞克隆和新产生的 与牛胰岛素反应以制备抗体的T细胞杂交瘤， 特异性识别T细胞免疫受体。 这些抗受体 然后将使用试剂来研究和调节生物化学， 伴随T细胞的抗原特异性活化的生理事件 并在最终的T细胞效应子功能中达到顶峰。 T细胞受体的生物化学和功能特性， 它调节的细胞内事件对我们理解 控制这种抗原特异性反应的因素 免疫调节细胞 免疫系统的异常调节 与I型糖尿病的病因学有关， 胰岛素“过敏”和胰岛素抗体的发展等问题。 描述免疫T细胞识别的机制和后果 将允许糖尿病的特异性免疫治疗策略。