FORMATION AND ELIMINATION OF SYNAPSES

突触的形成和消除

• 批准号: 3074675
• 负责人: CHIEN-PING KO
• 金额: $ 5.33万
• 依托单位: UNIVERSITY OF SOUTHERN CALIFORNIA
• 依托单位国家: 美国
• 财政年份: 1983
• 资助国家: 美国
• 起止时间: 1983-01-01 至 1987-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3074675
• 关键词: electrical potential; electrophysiology; freeze etching; fresh water environment; innervation; larva; membrane structure; myofibrils; nerve endings; nervous system regeneration; neural transmission; neuroanatomy; neuromuscular junction; neurotransmitters; pinocytosis; synapses

To understand how the nervous system operates it is important to study structure, function and formation of the synapse. One of the most important structure at the synapse is the site of transmitter release: the active zone. In this proposal the induction of the active zoe at ectopic endplate sites in the frog will be studied with intracellular recording and freeze-fracture electron microscopy. Ectopic junctions will be induced de novo by removing the original endplate zone and implanting original nerves to endplate-free areas. Whether or not the active zone is formed at the ectopic junction will be examined, especially in relation to the postjunctional fold if the latter is also formed. The result will be compared with a previous study on regeneration of the active zone at the original endplate. In addition the morphometry of active zones at ectopic junctions will be analyzed to study if it correlates with the increase in transmitter release shown with intracellular recording. The proposed research will elucidate how the unique organization of the active zone is induced and differentiated. It will also provide a better understanding on correlation between active zone structures and transmitter released efficacy. This basic knowledge on how synapses form and work would throw light on understanding the mechanisms of certain neurological diseases which may involve deficiency in transmitter release.

要了解神经系统是如何运作的，学习是很重要的