CORONARY BLOOD FLOW DEVELOPMENT--ROLE OF ADENOSINE

冠状动脉血流发展——腺苷的作用

• 批准号: 3082885
• 负责人: Gaynell PAUL MATHERNE
• 金额: $ 7.7万
• 依托单位: UNIVERSITY OF VIRGINIA
• 依托单位国家: 美国
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-09-01 至 1995-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3082885
• 关键词: adenosine; cyclic AMP; cyclic GMP; growth /development; guinea pigs; heart circulation; heart metabolism; high performance liquid chromatography; laboratory rabbit; mature animal; muscle cells; newborn animals; vascular endothelium; vascular smooth muscle

Birth and maturation are associated with dramatic alterations in blood flow regulation. One important modulator of blood flow is adenosine (ADO). However, little information exists about developmental changes in ADO modulation of blood flow. The central hypothesis of this investigation is that ADO modulation of coronary blood flow will change with development and the changes can be explained by cellular differences in ADO production and metabolism. To address this hypothesis we will accomplish the following: I.) Aims related to adenosine and local regulation of coronary flow. Aim IA: Determine adenosine production during development. Aim IB: Determine response to adenosine during development. II.) Aims related to cellular basis of developmental differences. Aim IIA: Determine ADO production by myocytes during development. Aim IIB: Determine ADO changes in cAMP and cGMP in vascular smooth muscle cells. Aim IIC: Determine uptake and transport of ADO in cardiac endothelial cells. Understanding the mechanisms of coronary flow regulation will provide new information about cardiac development and may provide information with clinical relevance to the management of premature infants and newborns with congenital heart disease.

出生和成熟与血液流动的剧烈变化有关。 监管。血液流动的一个重要调节剂是腺苷(ADO)。 然而，关于ADO的发展变化的信息很少 调节血液流动。这项调查的中心假设是 ADO对冠脉血流的调节作用将随着发展和 这些变化可以用细胞内ADO产生和释放的差异来解释 新陈代谢。为了解决这一假设，我们将完成以下工作： (一)与腺苷和冠脉血流局部调节相关的靶点。 目的IA：测定发育过程中腺苷的产生。 目的IB：测定发育过程中对腺苷的反应。 II.)目的与发育差异的细胞基础有关。 目的IIA：测定发育过程中心肌细胞产生ADO的情况。 目的IIB：测定血管平滑肌cAMP和cGMP的ADO变化 细胞。 目的IIC：测定心肌内皮细胞对ADO的摄取和转运 细胞。 了解冠脉血流调节的机制将提供新的 有关心脏发育的信息，并可提供以下信息 早产儿和新生儿高血压的临床意义 先天性心脏病。