DIAPHRAGM ADAPTATIONS TO RESPIRATORY TRAINING

呼吸肌疲劳与机械呼吸机依赖 极大地增加了患者的发病率和死亡率 患有慢性肺部疾病。横隔膜是指 主要的吸气肌和唯一的骨骼肌 被认为是重要的器官。然而，网络的适应能力 隔膜到不同量的呼吸功已收到 很少有调查关注。该项目的目标将是 定义生物化学特征和生物化学机制 大鼠横隔膜适应过度使用和停用；以确定 对废用的适应是可以预防或可逆的，并可获得 对生物化学局限性的新见解 呼吸肌疲劳和衰竭。这将会实现的 采用本申请者已在使用的实验模型。 具体地说，手术植入的气管电阻器被用来 研究横隔膜对耐力训练的反应 采用长时间机械通气法研究横隔膜 对废弃的反应。隔膜的自适应响应将 通过测量纤维类型、伸缩性和 耐力与整体机能生化指标 肌肉和线粒体氧化能力，初步数据 表明大鼠横隔肌确实适应了阻力 肥大训练适应机械通气 伴有萎缩。

Respiratory muscle fatigue and mechanical ventilator dependency contribute significantly to the morbidity and mortality of patients suffereing from chronic pulmonary disease. The diaphragm is the principal muscle of inspiration and the only skeletal muscle considered to be a vital organ. Yet, the adaptive capability of the diaphragm to varying amounts of respiratory work has received little investigative attention. The aim of this project will be to define the biochemical characteristics and mechanisms by which the rat diaphragm adapts to overuse and disuse; to determine if the adaptation to disuse is preventable or reversible and to gain new insight into the biochemical limitations responsible for respiratory muscle fatigue and failure. This will be accomplished employing experimental models already in use by this applicant. Specifically, surgically implanted tracheal resistors are used to study the diaphragm's response to endurance training and prolonged mechanical ventilation is used to study the diaphragm's response to disuse. The adaptive response of the diaphragm will be analyzed using measurements of fiber type, contractility and endurance as well as functional biochemical indices of whole muscle and mitochondria oxidative capacity, Preliminary data indicates that rat diaphragm muscle does adapt to resistance training with hypertrophy and adapts to mechanical ventilation with atrophy.