DIAPHRAGM ADAPTATIONS TO RESPIRATORY TRAINING

呼吸肌疲劳与机械通气依赖 显著增加患者的发病率和死亡率 患有慢性肺病。 隔膜是 吸气主肌和唯一的骨骼肌 被认为是一个重要的器官。 然而， 横膈膜对呼吸功的影响 很少的调查关注。 该项目的目的是 定义生物化学特征和机制， 大鼠横膈膜适应过度使用和废弃;以确定是否 对废用适应是可以预防或可逆的， 对生物化学限制的新见解 呼吸肌疲劳和衰竭。 这将是完成 采用本申请人已经使用的实验模型。 具体来说，手术植入的气管电阻器用于 研究横膈膜对耐力训练的反应， 长时间的机械通气被用来研究横膈膜的 对废弃的回应。 隔膜的自适应响应将 使用纤维类型、收缩性和 耐力和整体功能生化指标 肌肉和线粒体氧化能力，初步数据 表明大鼠膈肌确实适应阻力 适应机械通气 萎缩。

Respiratory muscle fatigue and mechanical ventilator dependency contribute significantly to the morbidity and mortality of patients suffereing from chronic pulmonary disease. The diaphragm is the principal muscle of inspiration and the only skeletal muscle considered to be a vital organ. Yet, the adaptive capability of the diaphragm to varying amounts of respiratory work has received little investigative attention. The aim of this project will be to define the biochemical characteristics and mechanisms by which the rat diaphragm adapts to overuse and disuse; to determine if the adaptation to disuse is preventable or reversible and to gain new insight into the biochemical limitations responsible for respiratory muscle fatigue and failure. This will be accomplished employing experimental models already in use by this applicant. Specifically, surgically implanted tracheal resistors are used to study the diaphragm's response to endurance training and prolonged mechanical ventilation is used to study the diaphragm's response to disuse. The adaptive response of the diaphragm will be analyzed using measurements of fiber type, contractility and endurance as well as functional biochemical indices of whole muscle and mitochondria oxidative capacity, Preliminary data indicates that rat diaphragm muscle does adapt to resistance training with hypertrophy and adapts to mechanical ventilation with atrophy.