MECHANISMS FOR CHANGES IN TOTAL LUNG CAPACITY IN HMD

HMD 中总肺活量的变化机制

• 批准号: 3082041
• 负责人: J CRAIG JACKSON
• 金额: $ 6.48万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 1986
• 资助国家: 美国
• 起止时间: 1986-07-01 至 1991-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3082041
• 关键词: Pongidae; atelectasis; electron microscopy; growth /development; histopathology; newborn animals; plethysmography; respiratory airway pressure; respiratory airway volume; respiratory distress syndrome of newborn

The area of research has been the sequence of changes in lung volumes and deflation stability during the first several days of life in premature monkeys with hyaline membrane disease (HMD). The candidate now seeks to expand his training in lung morphology and morphometry in order to provide a structural explanation for the observed changes in lung volume in animals with HMD. The research plan is designed to investigate the mechanisms for the fall in total lung capacity (TLC) during the development of HMD and for the increases observed during recovery. Preliminary experiments suggest that the fall in TLC may be due in part to atelectasis, but that other causes may be equally important. It is speculated that a better understanding of these other mechanisms, and the resolution of them during recovery, may provide important information about the abnormal repair processes that lead to bronchopulmonary dysplasia. The research plan proposes to measure TLC in prematurely delivered Macaca nemestrina monkeys with HMD and in controls. Animals will then be selected for sacrifice at three crucial states in the pathophysiology of HMD: as TLC is falling, after it has reached its nadir, and after return to normal. The inflated lung will then be quick frozen in situ, freeze dried, and examined by standard morphometric techniques. It is predicted that TLC will fall because of the development of airway debris and lung edema in addition to atelectasis; persistence of a decreased TLC may be caused, in part, by proliferation of type II pneumocytes. Research will be conducted within the research training environment of the Pediatric Pummonary Training Center and within the facilities of the Primate Center and the Perinatal Biology Laboratories at the University of Washington. Faculty with expertise in the pathophysiology of HMD, lung morphology and morphometry, and lung mechanics will supervise the research.

研究领域一直是肺体积和肺体积的变化顺序 早产儿出生后头几天的通缩稳定性 患有肺透明膜病(HMD)的猴子。这位候选人现在寻求 扩大他在肺形态和形态计量学方面的培训，以便提供 对观察到的动物肺体积变化的结构解释 头戴式头盔。 这项研究计划旨在调查坠落的机制 HMD发展过程中的总肺活量(TLC) 在复苏期间观察到的增加。初步实验表明， TLC下降可能部分是由于肺不张，但其他原因 可能同样重要。据推测，更好地理解 这些其他机制以及在恢复期间解决这些机制可能 提供有关导致异常修复过程的重要信息 到支气管肺发育不良。研究计划建议测量TLC 在早产的患有HMD和HMD的猕猴中 控制。然后动物将被选为祭品，在三个关键 HMD的病理生理状态：随着TLC的下降，在它 达到最低点，并在恢复正常后。充气的肺会 现场快速冷冻、冷冻干燥、达标检验 形态测量技术。据预测，TLC将会因为 除肺不张外，还出现呼吸道碎片和肺水肿； TLC下降的持久性可能部分是由于 II型肺泡细胞。 研究将在以下研究培训环境中进行 儿科肺科培训中心和设施内的 华盛顿大学灵长类中心和围产期生物学实验室 华盛顿。在HMD、肺部病理生理学方面有专业知识的学院 形态和形态计量学以及肺力学将指导这项研究。