MECHANISMS FOR CHANGES IN TOTAL LUNG CAPACITY IN HMD

HMD 中总肺活量的变化机制

• 批准号: 3082040
• 负责人: J CRAIG JACKSON
• 金额: $ 6.41万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 1986
• 资助国家: 美国
• 起止时间: 1986-07-01 至 1991-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3082040
• 关键词: Pongidae; atelectasis; electron microscopy; growth /development; histopathology; newborn animals; plethysmography; respiratory airway pressure; respiratory airway volume; respiratory distress syndrome of newborn

The area of research has been the sequence of changes in lung volumes and deflation stability during the first several days of life in premature monkeys with hyaline membrane disease (HMD). The candidate now seeks to expand his training in lung morphology and morphometry in order to provide a structural explanation for the observed changes in lung volume in animals with HMD. The research plan is designed to investigate the mechanisms for the fall in total lung capacity (TLC) during the development of HMD and for the increases observed during recovery. Preliminary experiments suggest that the fall in TLC may be due in part to atelectasis, but that other causes may be equally important. It is speculated that a better understanding of these other mechanisms, and the resolution of them during recovery, may provide important information about the abnormal repair processes that lead to bronchopulmonary dysplasia. The research plan proposes to measure TLC in prematurely delivered Macaca nemestrina monkeys with HMD and in controls. Animals will then be selected for sacrifice at three crucial states in the pathophysiology of HMD: as TLC is falling, after it has reached its nadir, and after return to normal. The inflated lung will then be quick frozen in situ, freeze dried, and examined by standard morphometric techniques. It is predicted that TLC will fall because of the development of airway debris and lung edema in addition to atelectasis; persistence of a decreased TLC may be caused, in part, by proliferation of type II pneumocytes. Research will be conducted within the research training environment of the Pediatric Pummonary Training Center and within the facilities of the Primate Center and the Perinatal Biology Laboratories at the University of Washington. Faculty with expertise in the pathophysiology of HMD, lung morphology and morphometry, and lung mechanics will supervise the research.

研究的领域是肺体积变化的顺序， 在早产儿出生后的头几天， 患有透明膜病（HMD）的猴子。 候选人现在寻求 扩大他在肺形态学和形态测量学方面的培训， 对观察到的动物肺体积变化的结构解释 关于HMD 该研究计划旨在调查秋季的机制， 总肺容量（TLC）在HMD的发展过程中， 在恢复期间观察到的增加。 初步实验表明， TLC下降可能部分是由于肺不张，但其他原因 可能同样重要。 据推测，更好地了解 这些其他机制，以及在恢复过程中解决这些机制， 提供有关异常修复过程的重要信息， 支气管肺发育不良 研究计划提出测量TLC 在患有HMD的早产猕猴中， 对照 然后在三个关键时刻选择动物进行牺牲。 在HMD的病理生理状态：随着TLC下降，在它已经 达到最低点后又恢复正常 膨胀的肺就会 就地速冻，冻干，标准检验 形态测量技术 据预测，TLC将下降， 除了肺不张之外，还出现气道碎片和肺水肿; TLC降低的持续性可能部分是由于 II型肺泡细胞。 研究将在研究培训环境中进行， 儿童抽水机培训中心和设施内的 灵长类动物中心和围产期生物学实验室， 华盛顿。 具有HMD病理生理学专业知识的教师，肺 形态学和形态测量学以及肺力学将监督研究。