REDOX EFFECTS IN A MODEL OF AORTIC TISSUE INJURY

主要目标是发展一个坚实的研究基础 与细胞生物学和生物化学相关的技术。 一阶段 培训，重点是开发组织损伤模型 以及表征局部氧化还原作用的初步研究 潜在的伤害和对伤害的反应，将在 卡尔Franzblau博士的实验室，系主任 生物化学。 第二阶段，这也将在博士的指导下完成。 Franzblau，将集中在临床相关问题的作用， 缺血缺氧时的氧化还原环境。 通过开发一种细胞培养模型， 可以评估损伤，可以 研究了 培养的平滑肌细胞，随着时间的推移，将形成一个多层的 细胞系统，其包含包埋在细胞外基质中的细胞， “丘陵和山谷”格式。 如果把这个模型比作最简单的 组织标本，假设细胞外的损伤 基质将激发细胞反应，导致快速、正常的修复。 在 努力找到一个建议的方法，一般控制，它是假设 局部氧化还原电位不仅在 伤害，也是对伤害的反应。 的边界内 培养系统中，将产生生物化学定义的基质， 反过来，可以以可控和可测量的方式受伤， 由嵌入基质中的细胞修复 初步研究已经 我很高兴这样的系统是可行的。 对该组织培养系统的仔细定义将允许 独立或依赖地发挥作用的评价 氧化还原电位和氧气在特定的情况下， 缺血和缺氧。 在第一阶段获得的技能 培训计划将使伯杰顿博士能够进一步完善这些技术 涉及测量和操纵氧化还原电位， 这些技术对平滑肌培养物以及其它细胞 类型，以研究当地的氧化还原环境是否可以显着 改变所造成的损害，包括功能性的和致命的，以及随后的 细胞对损伤的反应。

The major goal is the development of a solid foundation in research techniques related to cell biology and biochemistry. Phase I of the training, which will focus on the development of a model for tissue injury and initial studies characterizing the effects of local reduction-oxidation potentials on both injury and response to injury, will be conducted in the laboratory of Dr. Carl Franzblau, Chairman of the Department of Biochemistry. Phase II, which will also be done under the tutelage of Dr. Franzblau, will focus on the clinically relevant questions of the role of reduction-oxidation environments in ischemia and hypoxia. By developing a cell culture model in which tissue extracellular matrix injury may be assessed, various local physical chemical influences may be studied. Cultured smooth muscle cells, with time, will form a multilayered cell system containing cells embedded in an extracellular matrix in a "hills and valleys" format. If this model is likened to the simplest of tissue specimens, it is hypothesized that injury of the extracellular matrix will provoke cellular responses leading to rapid, normal repair. In an effort to find a proposed method of general control, it is postulated that the local reduction-oxidation potential plays a role not only in the injury but also in the response to that injury. Within the confines of the culture system, a biochemically defined matrix will be produced which, in turn, can be injured in a controlled and measurable manner and ultimately repaired by the cells embedded in the matrix. Preliminary studies have been encouraging that such a system is feasible. The careful definition of this tissue culture system will then allow for the evaluation of the roles played independently or dependently by reduction-oxidation potentials and oxygen in the particular scenario of ischemia and hypoxia. The skills acquired in the first phase of the training program will allow Dr. Bergethon to further refine the techniques relating to measuring and manipulating redox potentials and then apply these techniques to both the smooth muscle culture as well as other cell types in an effort to study if local redox environments can significantly alter the damage done, both functional and mortal, and the subsequent response of cells to the injury.