HUMORAL IMMUNE RESPONSE TO HPV 6 & 16 INFECTION

HPV感染的自然史有许多方面， 生殖道仍然知之甚少。 患病率估计值 HPV感染的检测是基于对病毒核酸的检测， 各种肛门生殖器部位的样本 我们开发了血清学检测方法 使用细菌表达的融合蛋白和合成肽， 能够在一种类型中测量人血清中HPV 6和16的抗体， 或“血清型”特异性方式。 该项目的总体目标是 获得关于HPV感染的体液免疫应答的数据。 的 具体目标是：1）确定HPV 6和16的患病率 母亲和他们的孩子，以及年轻人的抗体， 性活动; 2）确定是否有特征或 对各种病毒抗原的顺序反应，并确定是否 血清学特征可与临床状态相关;以及3） 确定体液抗体在调节免疫应答中起什么样的生物学作用， HPV感染，使用体外模型。

There are many aspects of the natural history of HPV infection of the genital tract that remain poorly understood. Estimates of the prevalence of HPV infection have been based on the detection of viral nucleic acids in samples from various anogenital sites. We have developed serologic assays using bacterially expressed fusion proteins and synthetic peptides which are capable of measuring human serum antibodies to HPV 6 and 16 in a type or "serotype" specific fashion. The overall goal of this project is to obtain data about the humoral immune response to HPV infection. The specific aims are: 1) to determine the prevalence of HPV 6 and 16 antibodies in mothers and their children, and in young adults initiating sexual activity; 2) to determine whether there is a characteristic or sequential response to various viral antigens, and determine whether the serological profile can be correlated with the clinical status; and 3) to determine what biological role the humoral antibodies play in modulating HPV infection, using in vitro models.