HUMAN PAPILLOMAVIRUS EXPRESSION AND ANTIGENICITY

该项目的总体目标是描述免疫反应， 生殖道HPV感染及免疫作用的界定 调节恶性肿瘤的进展。 血清学检测 针对HPV 6、16和18型的血清IgG抗体已经被开发并形成， 流行病学研究中HPV暴露的新标志物的基础。 的 本研究的具体目的是：1）继续表征体液 免疫反应，通过检查的贡献更深入 使用酵母表达的针对构象表位的抗体 使用重组牛痘病毒载体制备的蛋白质和病毒体;和 继续描述免疫反应表位; 2）检测HPV特异性抗原， 伊加抗体存在于血清、唾液和宫颈阴道分泌物中; 3） 为了比较Western印迹法与使用酵母表达的ELISA， 用于筛选方法的蛋白质、病毒粒子或合成肽; 4） 将对人群进行HPV抗体筛查，以确定a） 一般人群中抗体的流行率以及 肛门生殖器癌; B）与恶性肿瘤相关的免疫学标志物; c） 与血清阳性相关的危险因素; d）HPV与 抗体和其他感染措施;和5）研究细胞 在淋巴增殖试验中介导的对HPV感染的免疫应答， 通过识别细胞毒性T细胞。 关于免疫反应的信息 HPV感染在流行病学研究中是必不可少的， 疾病的管理，并在疫苗的开发，以防止 感染或肿瘤发展。

The overall goal of this project is to characterize the immune response to HPV infection of the genital tract and to define the role of immune modulation in the progression to malignancy. Serological assays to detect serum IgG antibodies to HPV types 6, 16 and 18 have been developed and form the basis for a new marker of HPV exposure in epidemiologic studies. The specific aims of this study are: 1) To continue characterizing the humoral immune response in greater depth by examining the contribution of antibodies directed against conformational epitopes using yeast expressed proteins and virions made using recombinant vaccinia virus vectors; and continuing to delineate immunoreactive epitopes; 2) To examine HPV specific IgA antibodies present in serum, saliva, and cervicovaginal secretions; 3) To compare the Western blot assay with ELISAs using yeast expressed proteins, virions, or synthetic peptides for screening methods; 4) Populations will be screened for HPV antibodies to determine a) the prevalence of antibodies in the general population and in cases with anogenital cancer; b) immunological markers associated with malignancy; c) risk factors associated with seropositivity; d) the correlation between HPV antibodies and other measures of infection; and 5) To study the cell mediated immune response to HPV infection in lymphoproliferative assays and by identifying cytotoxic T cells. Information about the immune response to HPV infection is essential in epidemiologic studies, in the clinical management of disease, and in the development of vaccines to prevent infection or tumor development.