HUMAN PAPILLOMAVIRUS EXPRESSION AND ANTIGENICITY

这个项目的总体目标是描述免疫应答的特征 人乳头瘤病毒在生殖道感染和免疫中的作用 恶变过程中的调节机制。血清学检测 针对HPV6型、16型和18型的血清免疫球蛋白抗体已经被开发并形成 在流行病学研究中建立新的HPV暴露标志物的基础。这个 这项研究的具体目的是：1)继续表征幽默 更深入的免疫反应，通过检查 利用酵母表达的针对构象表位的抗体 使用重组痘苗病毒载体制造的蛋白质和病毒粒子；以及 继续划定免疫反应表位；2)检测HPV特异性 血清、唾液和宫颈阴道分泌物中存在IgA抗体； 酵母表达产物的Western印迹试验与ELISAs的比较 用于筛选方法的蛋白质、病毒粒子或合成肽；4) 将对人群进行HPV抗体筛查，以确定a) 一般人群和非霍奇金淋巴瘤患者的抗体流行率 肛门生殖器癌；b)与恶性肿瘤有关的免疫学标志物；c) 与血清阳性相关的危险因素；d)人乳头瘤病毒与 抗体和其他感染措施；以及5)研究细胞 淋巴增殖试验中对HPV感染的介导性免疫反应 通过鉴定细胞毒性T细胞。有关免疫反应的信息 人乳头瘤病毒感染在流行病学研究中是必不可少的，在临床上 疾病管理，并在疫苗的开发中预防 感染或肿瘤发展。

The overall goal of this project is to characterize the immune response to HPV infection of the genital tract and to define the role of immune modulation in the progression to malignancy. Serological assays to detect serum IgG antibodies to HPV types 6, 16 and 18 have been developed and form the basis for a new marker of HPV exposure in epidemiologic studies. The specific aims of this study are: 1) To continue characterizing the humoral immune response in greater depth by examining the contribution of antibodies directed against conformational epitopes using yeast expressed proteins and virions made using recombinant vaccinia virus vectors; and continuing to delineate immunoreactive epitopes; 2) To examine HPV specific IgA antibodies present in serum, saliva, and cervicovaginal secretions; 3) To compare the Western blot assay with ELISAs using yeast expressed proteins, virions, or synthetic peptides for screening methods; 4) Populations will be screened for HPV antibodies to determine a) the prevalence of antibodies in the general population and in cases with anogenital cancer; b) immunological markers associated with malignancy; c) risk factors associated with seropositivity; d) the correlation between HPV antibodies and other measures of infection; and 5) To study the cell mediated immune response to HPV infection in lymphoproliferative assays and by identifying cytotoxic T cells. Information about the immune response to HPV infection is essential in epidemiologic studies, in the clinical management of disease, and in the development of vaccines to prevent infection or tumor development.