HUMORAL IMMUNE RESPONSE TO HUMAN PAPILLOMAVIRUS 6 AND 16 INFECTION

人乳头瘤病毒感染的自然历史有很多方面 生殖道仍然知之甚少。对流行率的估计 HPV感染的研究是基于对 从不同的肛门生殖部位采集的样本。我们已经开发出了血清学检测方法 使用细菌表达的融合蛋白和合成肽 能够检测人类血清中针对HPV6和16型的抗体 或“血清型”的特定时尚。这个项目的总体目标是 获取有关HPV感染的体液免疫反应的数据。这个 具体目标是：1)确定HPV6和16型的流行率 母亲及其子女的抗体，以及年轻人的抗体 性行为；2)确定是否有特征或 对各种病毒抗原的顺序反应，并确定是否 血清学特征可与临床状态相关；以及3) 确定体液抗体在调节 HPV感染，使用体外模型。

There are many aspects of the natural history of HPV infection of the genital tract that remain poorly understood. Estimates of the prevalence of HPV infection have been based on the detection of viral nucleic acids in samples from various anogenital sites. We have developed serologic assays using bacterially expressed fusion proteins and synthetic peptides which are capable of measuring human serum antibodies to HPV 6 and 16 in a type or "serotype" specific fashion. The overall goal of this project is to obtain data about the humoral immune response to HPV infection. The specific aims are: 1) to determine the prevalence of HPV 6 and 16 antibodies in mothers and their children, and in young adults initiating sexual activity; 2) to determine whether there is a characteristic or sequential response to various viral antigens, and determine whether the serological profile can be correlated with the clinical status; and 3) to determine what biological role the humoral antibodies play in modulating HPV infection, using in vitro models.