MUTAGEN FORMATION BY PYROLYSIS OF SOLID FUELS

在处理过程中产生的化合物的组成和致突变性 固体燃料的热解将在定义明确的 实验条件，以提名化合物更多 详细的生物学研究，并开发化学途径， 最令人担忧的化合物的形成。 选择 煤、木材和代表部分的模型化合物通常 在煤和木材中发现的物质将在流动装置中进行研究， 或在温度范围和时间内操作的床系统 常见的燃烧器类型。 产品将 分析，与核心实验室在分析 在化学上，我们把取代的， 未取代的、极性的和含氮的化合物， 生物测定 显示最大生物活性的亚组 将进行更详细的化学分析， 主要的致突变剂。 导致这些的化学途径 化合物将使用前线分子轨道理论进行评估 并通过用模型化合物进行的选择性实验。 的重点 研究将是氧和氮化合物，普遍存在于 煤和木材的不完全燃烧产物， 在有或没有酶激活的情况下引起细菌突变。

The composition and mutagenicity of compounds generated during the pyrolysis of solid fuels will be studied under well-defined experimental conditions in order to nominate compounds for more detailed biological studies and to develop chemical pathways for the formation of the compounds of greatest concern. Selected coals, woods, and model compounds representing moieties commonly found in coal and wood will be studied in either a flow apparatus or bed systems operated over temperature ranges and times encountered in common combustor types. The products will be analyzed, in collaboration with the core laboratory in Analytical Chemistry, to separate out major categories of substituted, unsubstituted, polar, and nitrogen-containing compounds for bioassay. The subgroups showing the greatest biological activity will be subjected to more detailed chemical analyses to identify the major mutagens. The chemical pathways leading to these compounds will be evaluated using frontier molecular orbital theory and by selected experiments with model compounds. The focus of the study will be on oxygen and nitrogen compounds, prevalent in the products of incomplete combustion of coal and wood and known to cause mutation of bacteria with or without enzymatic activation.