REGULATION OF HEART CELL FUNCTION BY MEMBRANE COMPONENTS

每一个细胞都被一层质膜所包围， 细胞外液，并将一个细胞与另一个细胞分开。 膜和 膜相关成分几乎在各个方面都起着重要作用 细胞功能。 在胚胎发育过程中， 多种组织型细胞特性与遗传相关 表面相关分子如受体 激素和神经递质，转运蛋白和离子传导 渠道 本计划的目标是了解 细胞膜的特定成分和膜相关的 细胞骨架作为细胞功能的调节器，以及这些细胞骨架的变化 组件与发展的结果，在实现明确的 可兴奋细胞的功能状态。 通过生物化学的应用， 生物物理，遗传和超微结构技术，心脏细胞在一个 一些体外模型系统，我们希望能够深入了解 膜功能的潜在机制。 方案的统一主题 是细胞表面成分在调节细胞功能中的作用。 那里 有三个主要的方法。 一个努力集中在特定的离子电导 心脏细胞膜中的机制;它们如何在胚胎中发育 心脏，以及它们在宏观和微观电学中的作用 活动 第二个焦点是胰岛素的受体机制， 乙酰胆碱，以及这些配体如何激活电流和其他细胞 活动 第三个领域是生物学的生理和遗传调节， 细胞膜中的离子运输机制。 这是一份续约提案 五年的支持，扩大努力，由七个经验丰富的 investigators. 该计划包括七个相互关联的项目， 六大核心设施

Every cell is surrounded by a plasma membrane that divides cytoplasm from extracellular fluid and separates one cell from another. The membrane and membrane-associated components play vital roles in virtually every aspect of cell function. During embryonic development, the differentiation of a variety of histotypic cell properties is associated with gentically controlled alterations in surface-associated molecules such as receptors for hormones and neurotransmitters, transport proteins and ion conductance channels. It is the goal of this Program to understand how certain specific components of the cell membrane and the membrane-associated cytoskeleton serve as regulators of cell function, and how changes in these components with development result in the achievement of definitive functional states in excitable cells. By application of biochemical, biophysical, genetic, and ultrastructural techniques to cardiac cells in a number of in vitro model system, we hope to gain insight into the mechanisms underlying membrane function. The unifying theme of the Program is the role of cell surface components in regulating cell function. There are three major approaches. One effort focuses on specific ion conductance mechanisms in the heart cell membrane; how they develop in the embryonic heart, and what role they have in macroscopic and microscopic electrical activity. A second focus deals with receptor mechanisms for insulin and acetylcholine, and how these ligands activate currents and other cellular activities. The third area is the physiological and genetic regulation of ion transport mechanisms in the cell membrane. This is a renewal proposal for five years of support for an expanded effort by seven experienced investigators. The Program includes seven inter-related projects subserved by six core facilities.