REGULATION OF HEART CELL FUNCTION BY MEMBRANE COMPONENTS

每个细胞都被一层质膜包围，质膜将细胞质与 细胞外液体，并将一个细胞与另一个细胞分开。膜和 膜相关成分几乎在各个方面都发挥着至关重要的作用。 细胞功能的变化。在胚胎发育过程中，细胞的分化 不同的组织类型细胞特性与基因相关 受体等表面相关分子的受控变化 激素和神经递质、转运蛋白和离子电导 频道。本计划的目标是了解如何确定 细胞膜的特定成分与膜相关 细胞骨架作为细胞功能的调节器，以及它们是如何变化的 具有开发成果的组件实现了最终的 可兴奋细胞的功能状态。通过应用生物化学， 生物物理、遗传学和超微结构技术在心脏细胞中的应用 多个体外模型系统，希望能对我们的研究有所了解 膜功能的潜在机制。节目的统一主题 是细胞表面成分在调节细胞功能中的作用。那里 有三个主要方法。其中一项工作集中在特定的离子电导上。 心脏细胞膜的机制；它们是如何在胚胎中发育的 心脏，以及它们在宏观和微观电学中的作用 活动。第二个焦点涉及胰岛素和胰岛素的受体机制。 乙酰胆碱，以及这些配体如何激活电流和其他细胞 活动。第三个方面是生理和遗传调节。 细胞膜中的离子传输机制。这是一份续签建议书 五年来支持由七位经验丰富的人 调查人员。该方案包括以下七个相互关联的项目 六个核心设施。

Every cell is surrounded by a plasma membrane that divides cytoplasm from extracellular fluid and separates one cell from another. The membrane and membrane-associated components play vital roles in virtually every aspect of cell function. During embryonic development, the differentiation of a variety of histotypic cell properties is associated with gentically controlled alterations in surface-associated molecules such as receptors for hormones and neurotransmitters, transport proteins and ion conductance channels. It is the goal of this Program to understand how certain specific components of the cell membrane and the membrane-associated cytoskeleton serve as regulators of cell function, and how changes in these components with development result in the achievement of definitive functional states in excitable cells. By application of biochemical, biophysical, genetic, and ultrastructural techniques to cardiac cells in a number of in vitro model system, we hope to gain insight into the mechanisms underlying membrane function. The unifying theme of the Program is the role of cell surface components in regulating cell function. There are three major approaches. One effort focuses on specific ion conductance mechanisms in the heart cell membrane; how they develop in the embryonic heart, and what role they have in macroscopic and microscopic electrical activity. A second focus deals with receptor mechanisms for insulin and acetylcholine, and how these ligands activate currents and other cellular activities. The third area is the physiological and genetic regulation of ion transport mechanisms in the cell membrane. This is a renewal proposal for five years of support for an expanded effort by seven experienced investigators. The Program includes seven inter-related projects subserved by six core facilities.