MID-ATLANTIC REGIONAL IVEM AND IMAGE ANALYSIS RESOURCE

大西洋中部地区 IVEM 和图像分析资源

• 批准号: 3104149
• 负责人: LEE D PEACHEY
• 金额: $ 26.17万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 1984
• 资助国家: 美国
• 起止时间: 1984-12-01 至 1992-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3104149
• 关键词: computer human interaction; computers; electron microscopy; image processing; stainings; tissue /cell preparation

A Regional Resource for Coordinated Intermediate Voltage Transmission Electron Microscopy (300-400 kV, IVEM) and Image Analysis will be established in Philadelphia to serve the mid-Atlantic region of the country. It is expected that sections of embedded cells and tissues up to about 5 micrometers in thickness and even thicker whole mounts of unembedded cells can be examined at these voltages. Images will be recorded either on film or digitized directly for image analysis. The emphasis in the image analysis will be on analysis in three dimensions. Core research will be on development of specimen preparation methods especially suited for study of cells and tissues by electron microscopy in this voltage range, including the development of stains selective for particular cell components. Image analysis core research will be directed toward developing automated data acquisition and efficient user-interactive image manipulation methods for extracting accurate three dimensional information, including morphometric information, from multiple images of structures, either serial micrographs or stereo tilt images. A variety of collaborative research projects is proposed. These projects and their sponsors will interact closely with the core research and the development of Resource capabilities. Letters of interest and intended use of the Resource have been received from users in New York, New Jersey, Pennsylvania, Maryland, and Washington, D.C. Training will be done on-site when users come to the facility, and hands-on workshops are planned for additional training and dissemination of information on the capabilities of the Resource. Further dissemination of results as well as capabilities of the Resource will be done through symposia organized by and/or at the Resource and through publication of research articles and review papers. The overall goal is to bring "state of the art" image processing capabilities into juxtaposition with a powerful tool for examining biological specimens of a wide variety of types and thicknesses in an environment of active and diverse biological research.

协调中压输电的区域资源