REGIONAL IVEM AND IMAGE ANALYSIS RESOURCE

这个实验室的目标是开发技术，以 定量的3D光学和电子显微镜，并使其可用 给科学界进行研究和教育。A 400千伏 透射电子显微镜允许检查样品10-20 比平时厚一倍，并已进行了修改，以实现无限范围的 用于3D成像的样品倾斜角度。一种激光扫描共聚焦光源 显微镜提供厚样品的光学切片，包括湿样品 和活标本，比用普通光线可以获得的标本更好 显微镜。核心研究的重点是量化、比较 使用不同成像模式的图像，以及对 制备方法和其他可能的人工制品来源的影响。 使用从其他导入的图像分析程序对图像进行处理 来源还是在这里开发的。程序可用于重建 从连续切片图像和以各种倾斜度拍摄的图像 (包括立体图像)。这些计划提供3D本地化 微粒标记物(金颗粒)、膜和 其他表面，以及网络的定量分析。最初，手动 实现了流程。我们计划在较长期内实现自动化， 尽可能多的进程。目前的合作研究项目有 专注于表面和表面受体分子的定位 在活动细胞中，细胞质基质的组织， 蛋白质网络的结构，横管的结构 骨骼肌中的纤维系统，以及 分化心肌和骨骼肌细胞。服务项目 涵盖广泛的主题。用户来自几个环境 来自美国和日本的。培训在设施现场进行，并且 通过在该设施和其他地方举办的讲习班。传播 关于该设施的信息是通过出版物、讲座 以及，在国家和国际会议上提交的海报，并直接 邮寄给生物医学科学家。

The objective of this laboratory is to develop techniques for quantitative 3D light and electron microscopy and to make these available to the scientific community for research and education. A 400 kV transmission electron microscope allows examination of specimens 10-20 times thicker than usual and has been modified for an unlimited range of specimen tilt angles for 3D imaging. A laser scanning confocal light microscope provides optical sectioning in thick specimens, including wet and living specimens, superior to that obtainable using ordinary light microscopes. The foci in the core research are quantitation, comparison of images using different imaging modalities, and the evaluation of the effects of preparation methods and other potential sources of artifacts. Images are processed using image analysis programs imported from other sources or developed here. Programs are available for reconstruction from serial section images and from images taken at various tilts (including stereo images). These programs provide 3D localization of particulate markers (gold particles), reconstruction of membranes and other surfaces, and quantitative analysis of networks. Initially, manual processes are implemented. We plan in the longer term to automate as many processes as possible. Current collaborative research projects are focussed on the localization of surfaces and surface receptor molecules in motile cells, the organization of the cytoplasmic matrix, the structure of protein networks, the structure of the transverse tubular system in skeletal muscle, and the development of the fibrillar system in differentiating cardiac and skeletal muscle cells. Service projects cover a broad range of topics. Users have come from several surrounding states and from Japan. Training is done on-site at the facility, and through workshops presented at the facility and elsewhere. Dissemination of information on the facility is done through publications, lectures and, posters presented at national and international meetings, and direct mailing to biomedical scientists.