REGIONAL IVEM AND IMAGE ANALYSIS RESOURCE

区域 IVEM 和图像分析资源

• 批准号: 2281647
• 负责人: LEE D PEACHEY
• 金额: $ 36.52万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 1984
• 资助国家: 美国
• 起止时间: 1984-12-01 至 1997-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2281647
• 关键词: biomedical equipment resource; confocal scanning microscopy; electron microscopy; image processing

The objective of this laboratory is to develop techniques for quantitative 3D light and electron microscopy and to make these available to the scientific community for research and education. A 400 kV transmission electron microscope allows examination of specimens 10-20 times thicker than usual and has been modified for an unlimited range of specimen tilt angles for 3D imaging. A laser scanning confocal light microscope provides optical sectioning in thick specimens, including wet and living specimens, superior to that obtainable using ordinary light microscopes. The foci in the core research are quantitation, comparison of images using different imaging modalities, and the evaluation of the effects of preparation methods and other potential sources of artifacts. Images are processed using image analysis programs imported from other sources or developed here. Programs are available for reconstruction from serial section images and from images taken at various tilts (including stereo images). These programs provide 3D localization of particulate markers (gold particles), reconstruction of membranes and other surfaces, and quantitative analysis of networks. Initially, manual processes are implemented. We plan in the longer term to automate as many processes as possible. Current collaborative research projects are focussed on the localization of surfaces and surface receptor molecules in motile cells, the organization of the cytoplasmic matrix, the structure of protein networks, the structure of the transverse tubular system in skeletal muscle, and the development of the fibrillar system in differentiating cardiac and skeletal muscle cells. Service projects cover a broad range of topics. Users have come from several surrounding states and from Japan. Training is done on-site at the facility, and through workshops presented at the facility and elsewhere. Dissemination of information on the facility is done through publications, lectures and, posters presented at national and international meetings, and direct mailing to biomedical scientists.

该实验室的目标是开发技术， 定量3D光学和电子显微镜，并使这些可用 用于科学研究和教育。 A 400 kV 透射电子显微镜允许检查标本10-20 倍比平常厚，并已修改为无限范围的 用于3D成像的样本倾斜角度。 一种激光扫描共焦光 显微镜可对厚标本（包括湿标本）进行光学切片 和活体标本，比普通光线下的效果好上级 显微镜. 核心研究的重点是定量、比较 使用不同成像模式的图像，以及 制备方法和其他潜在伪影来源的影响。 使用从其他网站导入的图像分析程序处理图像。 来源或开发。 可用于重建的程序 从连续切片图像和从在各种倾斜下拍摄的图像 （包括立体图像）。 这些程序提供3D本地化， 颗粒标记物（金颗粒），膜重建和 其他表面和网络的定量分析。 最初，手动 流程已实施。 从长远来看，我们计划自动化， 尽可能多的过程。 目前的合作研究项目有 专注于表面和表面受体分子的定位 在运动细胞中，细胞质基质的组织， 蛋白质网络的结构，横管的结构 骨骼肌中纤维系统的发育， 分化心肌和骨骼肌细胞。 服务项目 涵盖广泛的主题。 用户来自多个周边地区 国家和日本。 培训在工厂现场进行， 通过在该设施和其他地方举办的讲习班。 传播 关于该设施的信息是通过出版物，讲座 以及在国家和国际会议上展示的海报， 寄给生物医学科学家