WOUND HEALING AND WOUND INFECTION

伤口愈合和伤口感染

• 批准号: 3106064
• 负责人: THOMAS K. HUNT
• 金额: $ 84.19万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 1980
• 资助国家: 美国
• 起止时间: 1980-01-01 至 1994-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3106064
• 关键词: trauma; wound healing; wound infection

This proposal centers about the mechanism of wound healing: control and assurance of collagen synthesis, angiogenesis and resistance to infection, and the respective roles of some important during fetal life. The first project is based on our observation that one of the most powerful means which clinicians possess to enhance early wound healing and resistance to infection lies in the control of oxygen delivery via blood perfusing the injured tissue. Specific impediments to oxygenation have been detected and will be measured, and strategies to overcome them are proposed for testing. These studies extend our past investigations, and we expect them to lead to simple, inexpensive clinical measures which are likely to enhance repair and prevent infection. The second project deals with the control of collagen synthesis via effects of the metabolic microenvironment of wound on collagen gene transcription and posttranslational modification. The vital role of lactate in wounds is being correlated with the initiation of control of collagen synthesis and response to hormonal, growth factor stimuli. The third project deals with the expression of a growth factor, somatomedin (IGF I), its binding protein and a similar factor, platelet-derived growth factor, in wound healing. Particular reference is paid to the normal production of IGF in wounds, the interaction of IGF and its binding protein, as well as local modifications of these substance which affect repair. The fourth project is an exploration of the scar process in fetal wounds, which are remarkable for their ability to heal without scar. Control of scar, collagen synthesis and protease production, with particular regard to the unusually hypoxic nature of fetal wounds, will be explored. The core laboratory provides models of wound healing, collection and processing of wound fluid specimens from patients and animals, and tissue and cell culture facilities, as well as facilities for measuring oxygen, selected growth factors, lactate, pH and inflammatory mediators.

该建议的中心是伤口愈合的机制：控制和 保证胶原蛋白合成、血管生成和抗感染， 以及在胎儿生命中一些重要因素的各自作用。 第一个项目是基于我们的观察， 临床医生拥有的促进早期伤口愈合的方法， 对感染的抵抗力在于控制通过血液的氧气输送 灌注受伤的组织 氧化的具体障碍有 已经被发现并将被衡量，克服它们的战略是 建议进行测试。 这些研究扩展了我们过去的调查，我们 希望它们能带来简单、廉价的临床措施， 可能会增强修复并防止感染。 第二个项目涉及通过影响控制胶原蛋白的合成 创伤代谢微环境对胶原基因转录的影响 和翻译后修饰。 乳酸盐在伤口中的重要作用是 与胶原蛋白合成控制的起始相关， 对激素和生长因子刺激的反应 第三个项目涉及生长因子--生长调节素的表达 (IGF I），其结合蛋白和一个类似的因子，血小板衍生生长 因素，在伤口愈合。 特别提到的是正常的 伤口中IGF的产生，IGF的相互作用及其结合 蛋白质，以及这些物质的局部修饰， 修复. 第四个项目是探索胎儿伤口的疤痕过程， 它们能无疤痕愈合 控制 瘢痕、胶原蛋白合成和蛋白酶产生，特别是 胎儿伤口异常缺氧的性质，将被探讨。 核心实验室提供伤口愈合、收集和 处理来自患者和动物的伤口流体标本，以及组织 和细胞培养设施，以及测量氧气的设施， 选择的生长因子、乳酸盐、pH和炎症介质。