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A New Tool for Biochemical Analysis: Combining Enzyme-Responsive Gels with LCD Detection

生化分析新工具：酶响应凝胶与 LCD 检测相结合

基本信息

批准号：
BB/G014337/1
负责人：
Simon Webb
金额：
$ 35.51万
依托单位：
University of Manchester
依托单位国家：
英国
项目类别：
Research Grant
财政年份：
2010
资助国家：
英国
起止时间：
2010 至无数据
项目状态：
已结题

来源：
https://gtr.ukri.org/projects?ref=BB%2FG014337%2F1
关键词：
New Tool Biochemical Analysis Combining

项目摘要

Cells secrete a range of enzymes that allow them to modify their environment and facilitate their growth. However, the presence in these secretions of particular enzymes that breakdown or modify surrounding connective tissue can be prognostic markers of disease, e.g. the enzyme hyaluronidase, which is a clinically reliable marker for bladder cancer. In this proposal we describe a new methodology for the detection of biochemical events; combining enzyme-sensitive gels with liquid crystal displays. This proposal is a conceptual jump that builds upon a unique enzyme-sensing system we have developed, which combined liquid crystal display (LCD) technology (as found in LCD calculator screens) with an easy-to-manufacture bioanalyte surface. Our new concept exploits the three-dimensional and biocompatible 'semi-wet' nature of enzyme-sensitive gels; the gel is a reservoir of bioanalyte that provides the LCDs with mechanical stability (giving robust, portable sensors), whilst also supporting the growth of disease-causing cells on the sensor. These transparent enzyme-responsive gels will lend mechanical strength to arrays of LCDs constructed on transparent plastic chips; using these chips will make the LCDs visible to the naked eye. In response to secretions from disease-causing cells, these devices will show patterns of light and dark LCDs, which will profile enzymatic activity and reveal cellular markers of disease. Our system will make the identification of disease-causing enzymes and cells much faster, easier and cheaper, having the advantage over existing techniques in that it will give rapid results outside the laboratory without needing complex instrumentation.

细胞分泌一系列酶，使它们能够改变环境并促进生长。然而，这些分泌物中存在的分解或改变周围结缔组织的特定酶可能是疾病的预后标志，例如，透明质酸酶是临床上可靠的膀胱癌标志物。在本提案中，我们描述了一种检测生化事件的新方法；将酶敏感凝胶与液晶显示器相结合。该提案是一个概念上的跳跃，建立在我们开发的独特酶传感系统的基础上，该系统将液晶显示 (LCD) 技术（如 LCD 计算器屏幕）与易于制造的生物分析物表面相结合。我们的新概念利用了酶敏感凝胶的三维和生物相容性“半湿”性质；该凝胶是生物分析物的储存库，为 LCD 提供机械稳定性（提供坚固的便携式传感器），同时还支持传感器上致病细胞的生长。这些透明的酶响应凝胶将为透明塑料芯片上构建的液晶显示器阵列提供机械强度；使用这些芯片将使液晶显示器肉眼可见。为了响应致病细胞的分泌物，这些设备将显示明暗 LCD 图案，从而分析酶活性并揭示疾病的细胞标记。我们的系统将使致病酶和细胞的识别变得更快、更容易、更便宜，与现有技术相比，它的优势在于它可以在实验室外快速给出结果，而不需要复杂的仪器。