The role of the KRAB zinc-finger protein ZFP57 in the maintenance of the methylation programme in mouse development

KRAB 锌指蛋白 ZFP57 在维持小鼠发育中甲基化程序中的作用

• 批准号: BB/G020930/1
• 负责人: Anne Ferguson-Smith
• 金额: $ 46.68万
• 依托单位: University of Cambridge
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2009
• 资助国家: 英国
• 起止时间: 2009 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FG020930%2F1
• 关键词: role; KRAB; zinc; finger; protein

DNA is packaged inside our cells into bundles called chromosomes. Chromosome packaging is a highly regulated process that ensures that the correct cell makes the correct genes available for proper regulation specific to that cell. We are interested in the factors that regulate DNA packaging into chromosomes and in particular we are interested in the changes that happen to chromosome packaging as stem cells develop into body cells. We have made a mutant where key factors required for proper gene regulation are not placed on the DNA and the chromosomes. These experiments are designed to learn more about this key factor and how it influences the chromosomes in early development.

DNA在我们的细胞内被包装成一束束，称为染色体。染色体包装是一个高度调节的过程，确保正确的细胞使正确的基因可用于该细胞特异性的适当调节。我们对调节DNA包装成染色体的因素感兴趣，特别是我们对干细胞发育成体细胞时染色体包装发生的变化感兴趣。我们已经制造了一个突变体，其中正确基因调控所需的关键因子没有放置在DNA和染色体上。这些实验旨在更多地了解这个关键因素以及它如何影响早期发育中的染色体。

项目成果

Genomic properties of variably methylated retrotransposons in mouse.

• DOI: 10.1186/s13100-021-00235-1
• 发表时间: 2021-02-21
• 期刊: Mobile DNA
• 影响因子: 4.9
• 作者: Elmer JL;Hay AD;Kessler NJ;Bertozzi TM;Ainscough EAC;Ferguson-Smith AC
• 通讯作者: Ferguson-Smith AC

Variably methylated retrotransposons are refractory to a range of environmental perturbations.

• DOI: 10.1038/s41588-021-00898-9
• 发表时间: 2021-08
• 期刊: Nature genetics
• 影响因子: 30.8
• 作者: Bertozzi TM;Becker JL;Blake GET;Bansal A;Nguyen DK;Fernandez-Twinn DS;Ozanne SE;Bartolomei MS;Simmons RA;Watson ED;Ferguson-Smith AC
• 通讯作者: Ferguson-Smith AC

KRAB zinc finger protein diversification drives mammalian interindividual methylation variability.

• DOI: 10.1073/pnas.2017053117
• 发表时间: 2020-12-08
• 期刊: Proceedings of the National Academy of Sciences of the United States of America
• 影响因子: 11.1
• 作者: Bertozzi TM;Elmer JL;Macfarlan TS;Ferguson-Smith AC
• 通讯作者: Ferguson-Smith AC

A spontaneous genetically induced epiallele at a retrotransposon shapes host genome function.

• DOI: 10.7554/elife.65233
• 发表时间: 2021-03-23
• 期刊: eLife
• 影响因子: 7.7
• 作者: Bertozzi TM;Takahashi N;Hanin G;Kazachenka A;Ferguson-Smith AC
• 通讯作者: Ferguson-Smith AC

Metastable epialleles and their contribution to epigenetic inheritance in mammals

亚稳态表观等位基因及其对哺乳动物表观遗传的贡献

• DOI: 10.17863/cam.42457
• 发表时间: 2020
• 作者: Bertozzi T