LYMPHOID CELLS PRODUCTION OF ANTIBODIES

淋巴细胞产生抗体

• 批准号: 3115515
• 负责人: G. J THORBECKE
• 金额: $ 4.01万
• 依托单位: NEW YORK UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1984
• 资助国家: 美国
• 起止时间: 1984-04-01 至 1992-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3115515
• 关键词: B lymphocyte; T lymphocyte; antibody; antigens; cell differentiation; chickens; complement; delayed hypersensitivity; graft versus host disease; histocompatibility; immune tolerance /unresponsiveness; immunity; immunochemistry; immunologic memory; immunoregulation; laboratory mouse; lymphatic tissue

The function and fate of germinal center cells from lymphoid tissues will be analyzed in both mice and chickens. Particular attention will be given to kinetic events and their correlation to functional changes in B memory cells. Localization of radioactively labeled primed and unprimed B cells, and the influence of localized specific antigen on isolated primed B cell localization in recipients, will be followed both morphologically and functionally. Induction of B cell memory by injection of anti-idiotype alone or in the form of immune complex with idiotype will be tried in A/J mice, using the model of the major idiotype in the response to ARS in this strain. Athymic mice of A/J background will also be included. If such priming is obtained, the histological counterpart will be studied with respect to germinal center generation. Studies on acute antiidiotype formation during the response of mice to TNP on polysaccharide carriers are being continued with emphasis on its role in tolerance induction and on the role of idiotype specific T cells in this phenomenon. Experiments on B cell receptor blockade with TNP-polysaccharide antigens are being conducted in order to analyze the reversibility of such blockade procedures in terms of remaining responsiveness of the cells. The influence of infection with vesicular stomatitis virus on the induction of suppressor T cells as compared to helper T cells in vivo will be analyzed. Finally, the epidermal Langerhans cell is being studied and compared functionally to interdigitating reticulum cells of lymphoid tissue.

淋巴组织生发中心细胞的功能和命运将是 在小鼠和鸡中进行了分析。 将特别关注 动力学事件及其与 B 记忆细胞功能变化的相关性。 放射性标记的引发和未引发的 B 细胞的定位，以及 局部特异性抗原对分离的引发 B 细胞定位的影响 在接受者中，将在形态和功能上进行跟踪。 通过单独注射抗独特型抗体或以 具有独特型的免疫复合物将在 A/J 小鼠中进行试验，使用 该菌株对 ARS 反应的主要独特型。 A/J无胸腺小鼠 背景也将包括在内。 如果获得这样的启动，则组织学 将研究生发中心生成的对应物。 研究 小鼠对 TNP 反应期间急性抗独特型形成的影响 多糖载体正在继续强调其在 耐受诱导以及独特型特异性 T 细胞在此过程中的作用 现象。 TNP-多糖阻断B细胞受体的实验 正在进行抗原分析，以分析这种现象的可逆性 就细胞的剩余反应性而言，封锁程序。 这 水泡性口炎病毒感染对诱导的影响 将分析体内抑制性 T 细胞与辅助性 T 细胞的比较。 最后，正在研究和比较表皮朗格汉斯细胞 功能上与淋巴组织的指状网状细胞有关。