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LYMPHOID CELLS PRODUCTION OF ANTIBODIES

淋巴细胞产生抗体

基本信息

批准号：
3115516
负责人：
G. J THORBECKE
金额：
$ 12.22万
依托单位：
NEW YORK UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
1984
资助国家：
美国
起止时间：
1984-04-01 至 1992-02-28
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/3115516
关键词：
B lymphocyte T lymphocyte antibody antigens cell differentiation chickens complement delayed hypersensitivity graft versus host disease histocompatibility immune tolerance /unresponsiveness immunity immunochemistry immunologic memory immunoregulation laboratory mouse lymphatic tissue

项目摘要

The function and fate of germinal center cells from lymphoid tissues will be analyzed in both mice and chickens. Particular attention will be given to kinetic events and their correlation to functional changes in B memory cells. Localization of radioactively labeled primed and unprimed B cells, and the influence of localized specific antigen on isolated primed B cell localization in recipients, will be followed both morphologically and functionally. Induction of B cell memory by injection of anti-idiotype alone or in the form of immune complex with idiotype will be tried in A/J mice, using the model of the major idiotype in the response to ARS in this strain. Athymic mice of A/J background will also be included. If such priming is obtained, the histological counterpart will be studied with respect to germinal center generation. Studies on acute antiidiotype formation during the response of mice to TNP on polysaccharide carriers are being continued with emphasis on its role in tolerance induction and on the role of idiotype specific T cells in this phenomenon. Experiments on B cell receptor blockade with TNP-polysaccharide antigens are being conducted in order to analyze the reversibility of such blockade procedures in terms of remaining responsiveness of the cells. The influence of infection with vesicular stomatitis virus on the induction of suppressor T cells as compared to helper T cells in vivo will be analyzed. Finally, the epidermal Langerhans cell is being studied and compared functionally to interdigitating reticulum cells of lymphoid tissue.

淋巴组织生发中心细胞的功能和命运将是在小鼠和鸡身上进行了分析。我们将特别注意 B记忆细胞的动力学事件及其与功能变化的相关性。放射性标记启动和非启动B细胞的定位，以及特异性抗原对分离的预激B细胞定位的影响在接受者中，无论是在形态上还是在功能上都将被遵循。单独或联合注射抗独特型抗体诱导B细胞记忆独特型免疫复合物将在A/J小鼠身上进行试验，使用该菌株对ARS反应的主要独特型。A/J无性系小鼠背景资料也将包括在内。如果获得了这种启动，组织学上的对应者将就生发中心的生成进行研究。研究小鼠对TNP反应中急性抗独特型形成的研究多糖类载体继续被强调其在耐受诱导及独特型特异性T细胞在其中的作用现象。TNP-多糖阻断B细胞受体的实验研究正在进行抗原试验，以分析这种就细胞的剩余响应性而言，封锁程序。这个水泡性口炎病毒感染对水泡性口炎诱导的影响将分析体内抑制性T细胞与辅助性T细胞的比较。最后，对表皮朗格汉斯细胞进行了研究和比较。在功能上与淋巴组织的网状细胞交错。